Fabrication and evaluation of nanofibrous polyhydroxybutyrate valerate scaffolds containing hydroxyapatite particles for bone tissue engineering |
| |
| Authors: | Alireza Khoshraftar Fatemeh Yazdian Hamid Rashedi Roza Vaez Ghaemi Zakie Alihemmati |
| |
| Affiliation: | 1. Department of Biomedical Engineering, Islamic Azad University, Science and Research Branch, Yazd, Iran;2. Department of Life Science Engineering, Faculty of Interdisciplinary New Science and Technologies, University of Tehran (UT), Tehran, Iran;3. Department of Biotechnology, School of Chemical Engineering, College of Engineering, University of Tehran, Iran;4. Chemical and Biological Engineering Department, The University of British Columbia, Vancouver, Canada |
| |
| Abstract: | Tissue engineering is a new approach for regeneration of damaged tissues. The current clinical methods such as autograft and allograft transplantation are not effective for repairing bone damages, mainly due to the limited available sources and the donor-site side effects. In this research, the nanocomposite poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/nano hydroxyapatite (nHA) scaffolds with different nHA ratios for bone regeneration were utilized. The diameter and porosity of scaffolds were approximately 200?nm and 74%, respectively. The degradability test of the scaffolds suggests a low degradation rate with total degradation of 30% after 3 months. Cytotoxicity result showed that cultured osteoblast cells (MC3T3) on nanocomposite scaffolds had superiority in terms of higher proliferation and attachment in comparison with PHBV scaffold. The protein expression of alkaline phosphatase illustrated that nanofibrous scaffold containing hydroxyapatite had the highest alkaline phosphatase activities as a result of better proliferation. These results recommend that PHBV/nHA scaffolds are suitable candidates for bone tissue engineering. |
| |
| Keywords: | Bone electrospinning PHBV/nHA scaffold tissue engineering |
|
|
