Comparison of three-dimensional printing for fabricating silk fibroin-blended scaffolds |
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| Authors: | Kai Sun Ruixin Li Dong Li Wenxue Jiang |
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| Affiliation: | 1. Tianjin First Center Hospital, Department of Orthopaedic, Tianjin, China;2. Institute of Medical Equipment, Academy of Military and Medical Sciences, Department of Biomaterial, Tianjin, China;3. Tianjin Medical University General Hospital, Department of Orthopaedic, Tianjin, China |
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| Abstract: | In this study, two types of scaffolds were fabricated by 3D printing. Morphology, physical properties, biochemical were evaluated. Cell morphology and distribution were observed. It was found that the silk fibroin/collagen (SF/C) scaffold-based material had significantly higher values than the silk fibroin/chitosan (SF/CS) scaffold-based material. Hematoxylin and eosin staining of the scaffolds revealed that the number of cells in the SF/C scaffold was higher. Cells grew well inside the SF/C scaffold as measured by scanning electron microscope. Reverse Transcriotion-Polymerase Chain Reaction (RT-PCR) and Western blot showed that type II collagen and Sox9 can be found in SF/C scaffold. Therefore, the SF/C scaffold exhibited better overall performance compared with the SF/CS scaffold. |
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| Keywords: | 3D printing scaffold silk fibroin/chitosan silk fibroin/collagen tissue engineering |
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