Antimicrobial activity,effects on Streptococcus mutans biofilm and interfacial bonding of adhesive systems with and without antibacterial agent |
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| Affiliation: | 1. Student, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil;2. Chair, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil;3. Student, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil;4. Chair, Department of Physiological Sciences, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil;5. Department of Restorative Dentistry, School of Dentistry, University of Washington, Seattle, USA;6. Associate Professor, Department of Social Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil;7. Associate Professor, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil;1. Department of Prosthodontics, Faculty of Dentistry, Akdeniz University, Antalya, Turkey;3. Department of Endodontics, Faculty of Dentistry, Akdeniz University, Antalya, Turkey;2. Department of Restorative Dentistry, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey;1. Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, UNESP—Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil;2. Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, UNESP—Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil;3. Department of Physiology and Pathology, Araraquara School of Dentistry, UNESP—Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil;4. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, 1120 15th Street, CL-2112, Augusta, Georgia 30912-1129, USA;1. School of Dental Medicine, University of Zagreb, Gunduli?eva 5, 10000, Zagreb, Croatia;2. Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, Ki?pati?eva 12, 10000, Zagreb, Croatia;3. Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Gunduli?eva 5, 10000, Zagreb, Croatia;4. Department for Materials, Faculty of Mechanical Engineering and Naval Architecture, University of Zagreb, Ivana Lu?i?a 5, 10002 Zagreb, Croatia;5. Department of Oral Surgery, University Hospital Center Zagreb, School of Dental Medicine, University of Zagreb, Gunduli?eva 5, 10000, Zagreb, Croatia;1. Department of Prosthodontics, BezmialemVakif University, Faculty of Dentistry, Istanbul, Turkey;2. Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku and TYKS, University of Turku Hospital, Turku, 20520, Finland;3. Department of Prosthodontics, Faculty of Dentistry, Istanbul Medipol University, Istanbul, Turkey;4. Private Practicer, Dental Plus Clinic Bakirkoy, Istanbul, Turkey;1. Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea;2. Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, South Korea |
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| Abstract: | ObjectivesThis study evaluated the antimicrobial activity (by agar disk diffusion test, AD), viability of S. mutans biofilm (VB), and effect on resin-dentin interface (RDI) of six adhesive systems.MethodsThree adhesives containing antibacterial components (Gluma 2Bond (G2B)/glutaraldehyde, Clearfil SE Protect (CSP)/MDPB and Peak Universal Bond (PUB)/chlorhexidine) and the corresponding adhesives with similar composition, but without antibacterial agents (Gluma Comfort Bond, GCB; Clearfil SE Bond, CSB and Peak LC Bond, PLB) were evaluated. AD was determined measuring the extent of halo formation following application of adhesives and control groups (light cured or not) to cultures of four strict anaerobic and four facultative bacteria. For VB, a UA159 biofilm was grown on adhesive-coated hydroxyapatite discs for five days, collected and processed to count the number of viable cells. For RDI analysis, adhesives were applied according to manufacturers' recommendations and teeth were restored with resin composite, sectioned to obtain bonded slices and visualized by SEM.ResultsAn inhibition halo was observed for G2B (strict anaerobic/light cured and not light cured), CSP (strict anaerobic and facultative/light cured and not light cured) and PUB (strict anaerobic and facultative/not light cured). PUB when light cured produced an inhibition halo on L. casei and S. mutans only. G2B and CSP significantly reduced the viability of S. mutans. Adhesives containing antimicrobial compounds had no detectable effect on RDI.ConclusionThe MDPB-containing bonding agent showed better results of inhibition for all oral pathogens tested and a decrease of viability of Streptococcus mutans biofilm, among the adhesives tested. |
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| Keywords: | Antibacterial adhesives Dentine Hybrid layer |
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