In vitro Streptococcus mutans biofilm formation on surfaces of chlorhexidine-containing dentin bonding systems |
| |
| Affiliation: | 1. Department of Biomedical, Surgical and Dental Sciences, IRCCS Galeazzi Institute, University of Milan, via R.Galeazzi, 4, 20133 Milan, Italy;2. Department of Medical Sciences, Unit of Dental Sciences and Biomaterials, University of Trieste, Piazza dell’Ospitale, 1, 34129 Trieste, Italy;3. Department of Health Sciences, University of Milan, via Beldiletto, 1, 20142 Milan, Italy;4. Department of Oral Biology, College of Dental Medicine, Georgia Regents University, 1430 John Wesley Gilbert Dr., Augusta, GA 30912, USA;5. Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Via San Vitale, 59, 40125 Bologna, Italy;1. Department of Mechanical Engineering, University of Alberta, Edmonton T6G 2G8, Canada;2. Department of Mechanical and Aerospace Engineering, University of Central Florida, Orlando, FL 32816, USA;3. Department of Mechanical Engineering, Indian Institute of Science, Bangalore, India;1. Department of Environment Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;2. School of Materials Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;3. Lab Management and Service Center, University of Shanghai for Science and Technology, Shanghai 200093, China;1. National Engineering Research Center for Biomaterials and College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China;2. West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu 610041, China;1. Universidade Estadual do Oeste do Paraná (UNIOESTE), Departamento de Odontologia, ZIP 85819-110, Cascavel, Paraná, Brazil;2. Universidade Tecnológica Federal do Paraná (UTFPR), Departamento Acadêmico de Química, Bloco I4, ZIP 85884-000, Medianeira, Paraná, Brazil;1. Cariology and Operative Dentistry Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan;2. Research Fellow of Japan Society for the Promotion of Science, Tokyo Medical and Dental University, Tokyo, Japan;3. Cariology and Operative Dentistry Department, Tokyo Medical and Dental University, Tokyo, Japan;4. Endowed Department of International Oral Health Science, Tsurumi University, 2-1-3, Tsurumi, Tsurumi-ku, Kanagawa 230-0063, Japan;5. University of Hong Kong, Faculty of Dentistry, Hong Kong, China;1. School of Materials Science and Engineering, University of Science and Technology of China, Shenyang, 110016, China;2. Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China;3. Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, 110819, China |
| |
| Abstract: | This in vitro study evaluated the influence of chlorhexidine diacetate (CDA) when blended within dentin bonding systems (DBSs) on Streptococcus mutans (S. mutans) biofilm formation.One commercially available 0.2% wt CDA-containing DBS (Peak Universal Bond) and five experimental 0.2% wt CDA-containing DBS formulations (experimental Adper Scotchbond 1XT plus experimental resins, R2, R3, R4, R5) were assessed vs their no-CDA containing counterparts. Twenty-eight DBSs disks were prepared for each group (6.4 mm×1.0 mm) and cured for 80 s at 800 mW/cm2 in a nitrogen atmosphere. A modified Drip-Flow Reactor was used to grow S. mutans biofilms on specimen surfaces for 24 h and adherent, viable biomass was evaluated using a tetrazolium salt assay (MTT). Two specimens from each of the tested materials were processed with LIVE/DEAD stain and observed using laser confocal microscopy (CLSM) while two disks from each group were examined by using scanning electron microscopy (SEM).MTT assay, CLSM and SEM observations showed that CDA addition decreased, increased or did not change S. mutans biofilm formation. The lowest biofilm formation was obtained with Peak Universal Bond and R5 (with and without CDA).It may be concluded that the chemical composition of DBSs determines their ability to promote or hamper biofilm formation. Therefore, CDA addition may be helpful in modulating biofilm formation provided that DBS formulation is tuned and optimized. |
| |
| Keywords: | Antibacterial adhesives (A) Phosphate acid monomers (A) Confocal microscopy (C) Bioreactors |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
