Congo Red as a Supramolecular Carrier System for Doxorubicin: An Approach to Understanding the Mechanism of Action |
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| Authors: | Klaudia Kwieciń ska,Anna Stachowicz-Kuś nierz,Beata Korchowiec,Maciej Roman,Wojciech M. Kwiatek,Anna Jagusiak,Irena Roterman,Jacek Korchowiec |
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| Affiliation: | 1.Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland;2.Institute of Nuclear Physics, Polish Academy of Sciences, 31-342 Krakow, Poland;3.Faculty of Medicine, Medical College, Jagiellonian University, 31-034 Krakow, Poland;4.Department of Bioinformatics and Telemedicine, Faculty of Medicine, Medical College, Jagiellonian University, 30-688 Krakow, Poland |
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| Abstract: | The uptake and distribution of doxorubicin in the MCF7 line of breast-cancer cells were monitored by Raman measurements. It was demonstrated that bioavailability of doxorubicin can be significantly enhanced by applying Congo red. To understand the mechanism of doxorubicin delivery by Congo red supramolecular carriers, additional monolayer measurements and molecular dynamics simulations on model membranes were undertaken. Acting as molecular scissors, Congo red particles cut doxorubicin aggregates and incorporated them into small-sized Congo red clusters. The mixed doxorubicin/Congo red clusters were adsorbed to the hydrophilic part of the model membrane. Such behavior promoted transfer through the membrane. |
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| Keywords: | doxorubicin, Langmuir films, drug– membrane interactions, Raman spectroscopy, molecular dynamics simulations, drug carrier |
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