高压液相色谱法测定食品中的黄曲霉毒素Bl

本文应用HPLC-FLD,在激发波长365nm、荧光波长450nm,流动相为甲醇/水1∶1、流速1.0ml/min,色谱柱为Hypersul,ODS5μm、125×4mm条件下,测定黄曲霉毒素B1衍生物-黄曲霉毒素B2a[1],得到AFTB1浓度与峰面积成正比的工作曲线(相关系数r=1.000).本法精密度、准确度好,灵敏度高,其变异系数CV为2.5%,回收率范围为95%～101%,检出限为0.2ng.

Determination of Aflatoxin B1 in Food with HPLC

In the present study,HPLCFLD was used to examine the aflatoxin B1 derivativeB2ain food,with excitation and fluorescence wave-lengths 365nm and 450nm respectively,methanol/H2O=11 in the mobile phase and a flow rate 10ml/min,1254mm Hypersil column packed with ODS5um. A working curve showing a direct ratio (r=1.000) of the concentration against the peak area of AFTB1 was obtained.The method proved to be satisfactory in precision,accuracy and sensitivity,with CV=25%,recovery rate 95%￣101% and detection limit 02ng.