Rapid degradation of the mycotoxin patulin in man quantified by stable isotope dilution assays |
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Authors: | Michael Rychlik |
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Affiliation: | Institut für Lebensmittelchemie der Technischen, Universit?t München, Lichtenbergstr. 4 D-85748, Garching, Germany. michael.rychlik@ch.tum.de |
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Abstract: | The absorption and degradation of the mycotoxin patulin in man was quantified by using a recently developed stable isotope dilution assay. Application of this currently most sensitive method revealed a patulin content less than 200 ng l -1 in the blood serum of five consumers of apple juice. Likewise, no patulin was found in the serum of a volunteer, whose blood was drawn shortly after consumption of a juice containing a maximum tolerable amount of patulin. In further in vitro experiments, the degradation of patulin by reacting it with whole blood was investigated. After addition of 100 μg patulin to 9 ml blood, only 6.1% of the mycotoxin was detected after 2 min. It was concluded, therefore, that even high naturally occurring concentrations of patulin in foods are quickly degraded before reaching other tissues than the gastrointestinal tract. |
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Keywords: | Glutathione Human Study Patulin Absorption Stable Isotope Dilution Assay |
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