| 高效液相色谱法测定苦瓜啤酒中亮蓝 |
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| 引用本文： | 龙洲雄,胡海山,万春花,鄢兵. 高效液相色谱法测定苦瓜啤酒中亮蓝[J]. 食品科学, 2006, 27(12): 657-659 |
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| 作者姓名： | 龙洲雄 胡海山 万春花 鄢兵 |
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| 作者单位： | 江西省分析测试研究所； |
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| 摘 要： | 本文采用聚酰胺粉吸附提取苦瓜啤酒中的色素亮蓝。用HPLC法测定,采用KromasilC18柱,乙腈-0.02mol/L乙酸铵溶液为流动相,梯度洗脱,流速1.0ml/min,检测波长627nm。回收率在89.6%～97.6%之间,RSD(n=6)为3.25%,最低检出浓度为0.002mg/kg。实验结果表明该法快速、简便、准确可靠。
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| 关 键 词： | 高效液相色谱 苦瓜啤酒 亮蓝 |
| 文章编号： | 1002-6630（2006）12-0657-03 |
| 收稿时间： | 2006-09-20 |
| 修稿时间： | 2006-09-20 |
Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography |
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| LONG Zhou-xiong,HU Hai-shan,WAN Chun-hua,YAN Bing. Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography[J]. Food Science, 2006, 27(12): 657-659 |
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| Authors: | LONG Zhou-xiong HU Hai-shan WAN Chun-hua YAN Bing |
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| Affiliation: | Jiangxi Institute of Analysing and Testing, Nanchang 330029, China |
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| Abstract: | An HPLC method was developed to determine bright blue in bitter gourd beer. After being extracted with polyamide resin, bright blue could be adsorbed on Kromasil C18 column, and eluted gradiently by using acetonitrile -0.02mol/L ammonium acetate as the mobile phase at flow rate 1.0ml/min, and be detected at 627nm. The recovery of sample is in the range of 89.6%～ 97.6%, RSD(n=6) is 3.25%. This method is proved to be simple, rapid and accurate . |
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| Keywords: | HPLC bitter gourd beer bright blue |
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