Detection of verocytotoxin-producing Escherichia coli (VTEC) in minced beef and raw milk by colony blot hybridization |
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| Affiliation: | 1. Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, U.O. Putignano, V. Chiancolla n. 1, Putignano, BA, Italy;2. Università di Bari, Facoltà di Medicina Veterinaria, Dip. Sanità e Benessere Animale, S.P. per Casamassima Km 1.3, Valenzano, BA, Italy;1. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China;2. Department of Environmental & Global Health, College of Public Health & Health Professions, and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA;3. Department of Disease Prevention and Control, Wuxi Center for Disease Control and Prevention, Wuxi 214023, PR China;1. Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada;2. Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada;1. Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA, USA;2. Department of Oral Surgery and Oral Medicine, Faculty of Dentistry, University of Oslo, Oslo, Norway;3. Mercy Ships, Lindale, TX, USA;1. Department of Agronomy, Telidji Amar University, BP 37G, Ghardaïa Road, 03000 Laghouat, Laboratory of Biotechnology Related to Animal Breeding, University Saad Dahleb, BP: 270, Soumaa Road, Blida, Algeria;2. Higher National Veterinary School of Algiers, BP 161 Hacène Badi El Harrach, Algiers, Algeria;3. Institute of Veterinary Sciences, Laboratory of Biotechnology Related to Animal Breeding, University Saad Dahleb, BP: 270, Soumaa Road, Blida, Algeria;1. Unit 92919 of PLA, Ningbo, Zhejiang 315020, China;2. Business School, Sichuan University, Chengdu, Sichuan 610064, China;3. The 28th Research Institute of CETC, Nanjing, Jiangsu 210007, China;1. Divisão de Reumatologia, Departamento de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brasil;2. Divisão de Nefrologia, Departamento de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brasil |
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| Abstract: | Verocytotoxin-producing Escherichia coli (VTEC) are foodborne pathogens that cause outbreaks linked to consumption of meat and raw milk. In this note the authors report results obtained from a survey conducted on minced beef and raw bovine milk samples using a Multiplex PCR (M-PCR) for the detection of eae, stx1, stx2 and hlyA genes as a screening step followed by a colony blot hybridization (CBH) technique for the isolation of the VTEC. Of 100 minced beef and 123 raw milk samples, 13 (13%) and 7 (5.7%) were positive in the M-PCR and among these 9 and 3 strains were isolated using CBH, respectively. All isolates showed the presence of the stx2 gene, single or in association with the other investigated genes. None of the isolates belonged to the O157, O26, O91, O103, O111 and O145 serogroups. The study showed that the use of M-PCR for the screening of samples coupled with a sensitive and specific detection technique, could improve the possibility of detection of VTEC strains in foods. Moreover, the presence of VTEC in minced beef and in raw milk confirms their important role as putative vehicles of infection to humans. Stringent control of these foodstuffs is essential for food safety purposes. |
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