Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor |
| |
| Affiliation: | 1. Nanobio Engineering Laboratory, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;2. Bioinnovare Co., Ltd., 5-5-531-722, Koyo-cho Naka, Higashinada-ku, Kobe 658-032, Japan;1. Nanyang Technological University, NEW-CREATE Programme, 1 CREATE Way, Research Wing, #02-06/08, Singapore 138602, Singapore;2. Dipartimento di Scienze AgroAlimentari, Ambientali e Animali, University of Udine, via delle Scienze 206, 33100 Udine, Italy;3. Nanyang Technological University-Hebrew University of Jerusalem-Ben Gurion University (NEW-CREATE) Programme, 1 CREATE Way, Research Wing, #02-06/08, Singapore 138602, Singapore;4. Department of Biotechnology Engineering, Ben-Gurion University of the Negev, PO Box 653, 84105 Beer-Sheva, Israel;1. Nanotechnology & Catalysis Research Centre, (NANOCAT), University of Malaya, Block 3A, Institute of Postgraduate Studies Building, Kuala Lumpur 50603, Malaysia;2. Department of Chemistry, University of Malaya, and also Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia;1. Biosensors and Biotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410, Brunei Darussalam;2. Institute of Forestry and Environmental Sciences, University of Chittagong, Chittagong 4331, Bangladesh;1. Bioengineering and Sensing Technology Resear?ch Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand;2. Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand;3. Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand;4. Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand;5. Center of Excellence on Environmental Health and Toxicology, Ministry of Education, Bangkok 10400, Thailand;1. Biosensors and Biotechnology Laboratory, Chemical Science Programme, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, BE 1410 Brunei Darussalam;2. Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA, USA;1. Forensic Science Program, Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Thailand;2. Forensic Science Innovation and Service Center, Prince of Songkla University, Thailand;3. Division of Physical Science, Faculty of Science, Prince of Songkla University, Thailand;4. Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Thailand;5. Thailand Center of Excellence in Physics, Commission on Higher Education, Bangkok, Thailand;6. Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Thailand |
| |
| Abstract: | An easy, rapid and sensitive method of detection of the presence of meat species in raw or processed foods is important from cultural, religious, health and commercial perspectives. In this study we have tried to distinguish species-specificity in control and processed pork, chicken and bovine meats using loop mediated isothermal amplicons (LAMP) and disposable electrochemical printed (DEP) chips. LAMP is a nucleic acid amplification method that amplifies target DNA with high specificity, efficiency and rapidity under isothermal condition (63 °C). Electrochemical genosensor with the DEP chips detects the amplicons by Linear Sweep Voltammetry (LSV) observation of DNA–Hoechst33258 interaction on the chip surface. Hoechst33258 interacts with DNA in solution without immobilization of DNA onto the electrode surface eliminating the time consuming probe immobilization step. Our method is more specific and free of unwanted amplifications compared to Multiplexed PCR (M-PCR) method and gave limits of detection of ～20.33 ng/μl (3 × 104 copies/reaction), ～78.68 pg/μL (3 × 102 copies/reaction) and ～23.63 pg/μL (30 copies/reaction) for pork, chicken and bovine species, respectively. Our method of detection is quick, taking only an hour, and it may be useful for food administration laboratories to carry out meat species identification in raw and processed foods. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
