Study on the Sliding Friction of Endothelial Cells Cultured on Hydrogel and the Role of Glycocalyx on Friction Reduction |
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| Authors: | Yong Mei Chen Takayuki Kurokawa Taiki Tominaga Kazunori Yasuda Yoshihito Osada Jian Ping Gong Kimiko Yamamoto Joji Ando |
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| Affiliation: | 1. Faculty of Advanced Life Science, Hokkaido University, Sapporo 060‐0810, Japan;2. MOE Key Laboratory for Non‐equilibrium Condensed Matter and Quantum Engineering, Department of Chemistry, School of Science, Xi'an Jiaotong University, Xi'an 710049, PR China;3. Present address: RIKEN 2‐1, Hirosawa, Wako, Saitama 351‐0198, Japan;4. School of Medicine, Hokkaido University, Sapporo 060‐0810, Japan;5. Graduate School of Medicine, University of Tokyo, Tokyo 113‐0033, Japan |
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| Abstract: | We investigate the sliding friction of HUVEC monolayers cultured on PNaSS gel, intending to elucidate the role of glycocalyx on the surface of ECs in friction reduction. Three sets of HUVEC monolayers are investigated: an as‐cultured HUVEC monolayer; a HUVEC monolayer treated with TGF‐β1, which increases the glycocalyx by 148%; and a HUVEC monolayer treated with heparinase I, which reduces the glycocalyx by 57%. When being slid on a flat, glass surface, the frictional stress of the HUVEC monolayer decreases in the order: heparinase‐I‐treated > as‐cultured > TGF‐β1‐treated samples. The results suggest that glycocalyx may play a role in reducing the friction of endothelial cell monolayer. |
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