维生素A对小鼠免疫调节功能的影响

目的探讨维生素A对小鼠免疫调节功能的影响。方法人工制备低维生素A、正常维生素A、高维生素A3种饲料,分别按常规饲养3组小鼠5周,应用微量荧光法测定血清维生素A浓度,三色流式细胞术测定CD4+T细胞内细胞因子IL-4和IFN-γ水平。结果3组小鼠血清维生素A浓度差异有显著意义,低维生素A组IFN-γ水平(28.60%±10.82%)明显高于正常对照组(20.13%±6.39%);高维生素A组IL-4水平(5.31%±2.04%)明显高于正常对照组(2.80%±1.37%),差异有非常显著意义(均P<0.01)。随着维生素A水平的提高,IFN-γ表达率降低,Th1免疫应答减弱,IL-4表达率增高,Th2免疫应答增强。结论维生素A对小鼠Th1/Th2免疫应答影响显著。

Influence of Vitamin A on Immunoregulatory Function of Mice

Objective To explore the influence of vitamin A on the immunoregulatory function of mice.Methods Twenty-four mice were divided into three groups and fed by the prepared forages with low,normal and high vitamin A contents for 5 weeks respectively,then determined for serum vitamin A concentration by microfluorescence spectrophotometry,and for the IL-4 and IFN-γ levels in CD4+T cells by trichromatic flow cytometry.Results Significant difference was observed in the serum vitamin A concentrations of mice in the three groups.However,the IFN-γ level of mice fed by the forage with low vitamin A content(28.60%±10.82%) was significantly higher than that with normal vitamin A content(20.13%±6.39%).The IL-4 level of mice fed by the forage with high vitamin A content(5.31%±2.04%) was significantly higher than that with normal vitamin A content(2.80%±1.37%). Along with the increasing content of vitamin A in forage,the IFN-γ expression and Th1 immune response level decreased,and the IL-4 expression and Th2 immune response level increased.Conclusion Vitamin A showed significant influence on the Th1/Th2 immune response of mice.