| Bacillus Mycoides磷脂酶C部分特性研究 |
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| 引用本文： | 王常高,刘彦昌,干信,陈涛.Bacillus Mycoides磷脂酶C部分特性研究[J].化学与生物工程,2007,24(7):52-54. |
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| 作者姓名： | 王常高 刘彦昌 干信 陈涛 |
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| 作者单位： | 1. 湖北工业大学生物工程学院湖北省工业微生物重点实验室,湖北,武汉,430068
2. 中国科学院武汉病毒研究所,湖北,武汉,430071 |
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| 基金项目： | 湖北省自然科学基金
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湖北省教育厅科研项目
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湖北工业大学科技项目 |
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| 摘 要： | 对一种纯化自Bacillus mycoides 970发酵上清液的磷脂酶C的部分特性进行了研究.SDS-PAGE检测表明该纯化磷脂酶C由一条多肽链组成,分子量为75.1 kDa.热稳定性实验表明该纯化磷脂酶C对温度敏感,具有热不稳定性.金属离子依赖性检测表明该纯化磷脂酶C属于金属磷脂酶C家族,Ca2 、Mg2 、Zn2 和Ba2 对该磷脂酶C催化活性的发挥必不可少.血平板及卵黄平板检测表明该磷脂酶C没有溶血活性和卵磷脂酶活性.
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| 关 键 词： | 磷脂酶C Bacillus mycoides 特性 |
| 文章编号： | 1672-5425（2007）07-0052-03 |
| 修稿时间： | 2007-03-30 |
Study on Some Properties of Phospholipase C from Bacillus Mycoides |
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| WANG Chang-gao,LIU Yan-chang,GAN Xin,CHEN Tao.Study on Some Properties of Phospholipase C from Bacillus Mycoides[J].Chemistry & Bioengineering,2007,24(7):52-54. |
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| Authors: | WANG Chang-gao LIU Yan-chang GAN Xin CHEN Tao |
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| Affiliation: | 1. Hubei Key Laboratory of Industrial Microorganisms ,College of Bioengineering, Hubei University of Technology, Wuhan 430068, China ; 2. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China |
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| Abstract: | Some properties of phospholipase C(PLC) from Bacillus mycoides 970 are studied in this paper.The PLC is indicated to be a single polypeptide and its molecular mass is estimated to be 75.1 kDa by the SDS-PAGE.The PLC is sensitive to temperature and is heat-labile.The PLC belongs to a group of metallophospholipase C and Zn2 ,Ca2 ,Mg2 and Ba2 are essential for its hydrolyzing activity.The enzyme shows no lecithinase activity on egg yolk agar and no hemolytic activity to human erythrocytes on blood agar. |
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| Keywords: | phospholipase C Bacillus mycoides property |
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