流行性乙型脑炎病毒减毒株at222的生物学特性

目的 分析乙型脑炎病毒减毒株ａｔ２２２生物学特性，并与亲代野生强毒株ＡＴ２１进行比较。方法 ａｔ２２２株和 ＡＴ３１株在伊蚊细胞Ｃ６／３６上繁殖，培养上清用作病毒液，在Ｖｅｒｏ细胞上以病毒蚀斑法测定病毒滴度，以 小鼠脑内感染法测定其神经毒力。结果ａｔ２２２株在蚊细胞上生长缀慢，但仍可达到与其亲代强毒株相似的滴度， 即ｌ０８ＰＦＵ／ｍｌ。在Ｖｅｒｏ细胞上形成蚀斑比其亲代野生强毒株ＡＴ３１明显小，脑内感染小鼠不致死，而ＡＤＩ株却表现 出了极强的神经毒力。结论 对乙型脑炎病毒减毒株出ａｔ２２２的生物学特性进行了全面分析，为研究和开发乙型脑炎 疫苗提供理论依据。

Biological Characteristics of Attenuated Strain at222 of Japanese Encephalitis Virus

Objective To analyze and compare the biological characteristics of attenuated Japanese encephalitis virus strain at222 and its parental strain AT31 .Methods AT31 and at222 strains were propagat-ed in mosquito C6/36 cells, and the virus tilers and neurovirulences of them in supernatant were detected by plaque assay on Vero cells and intracerebral test in mice respectively. Results at222 strain grew slowly in mosquito cells,however, the titer(108PFU/ml)of it was almost identical to that of its parental strain AT31 .The plaques of at222 strain on Vero cells were significantly smaller than those of strain AT31 .The intracerebral test in mice also showed low neurovirulence of at222 strain. Conclusion The biological characteristics of at222 stain are cmopletely analyzed in this paper.The study provides a basis in theory for developing JE vaccine.