| 乳酸菌活性物质LPX-600的分离纯化及其性质的研究 |
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| 引用本文： | 徐洪涛,权春善,刘长建,王军华,范圣第. 乳酸菌活性物质LPX-600的分离纯化及其性质的研究[J]. 食品与发酵工业, 2005, 31(9): 127-130 |
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| 作者姓名： | 徐洪涛 权春善 刘长建 王军华 范圣第 |
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| 作者单位： | 1. 大连理工大学环境与生命学院,大连,116024
2. 大连民族学院,生命科学学院,大连,116600 |
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| 基金项目： | 辽宁省教育厅基金项目（2004F086） |
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| 摘 要： | 经硫酸铵沉淀、阳离子交换层析、凝胶过滤、反相高效液相色谱等分离纯化过程,从类植物乳杆菌(Lactobacillus paraplantarum LPX-600)的发酵液中分离出1种活性物质LPX600。LPX600在酸性条件下活性较高,对热稳定,对蛋白酶K、胃蛋白酶、木瓜蛋白酶不敏感,并且对沙门氏菌、变形杆菌、金黄色葡萄球菌、粘性沙雷氏菌等食品致病菌有很强的抑菌活性。
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| 关 键 词： | 类植物乳杆菌 分离纯化 抑菌活性 |
| 收稿时间： | 2005-04-23 |
| 修稿时间： | 2005-04-23 |
Characterization and Purification of LPX-600 by Lactobacillus paraplantarum LPX-600 |
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| Xu Hongtao,Quan Chunshan,Liu Changjian,Wang Junhua,Fan Shengdi. Characterization and Purification of LPX-600 by Lactobacillus paraplantarum LPX-600[J]. Food and Fermentation Industries, 2005, 31(9): 127-130 |
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| Authors: | Xu Hongtao Quan Chunshan Liu Changjian Wang Junhua Fan Shengdi |
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| Affiliation: | 1.Dalian University of Technology, Dalian, 116024, Chin;2.Dalian Nationalities University, Dalian, 116600, China |
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| Abstract: | Inhibitory substance LPX-600 produced by Lactobacillus paraplantarum LPX-600 was purified by ammonium sulfate precipitation, cation-exchange chromatography, gel filtration and reverse-phase HPLC. The study showed that LPX-600 was heat-stable, active at pH<6.0, not sensitive to proteolytic enzymes (protease K, pepsin, papain). It strongly inhibits many food borne bacteria such as Salmonella sp., Staphylococcus aureus, Serratia marcescens, and Proteus vulgaris. |
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| Keywords: | Lactobacillus paraplantarum purification inhibitory activity |
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