| 水菖蒲内生菌分离与抗菌活性菌株筛选 |
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| 引用本文： | 周晓坤,陈钧,韩邦兴,何佳,刘长风.水菖蒲内生菌分离与抗菌活性菌株筛选[J].食品科学,2010,31(9):211-215. |
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| 作者姓名： | 周晓坤 陈钧 韩邦兴 何佳 刘长风 |
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| 作者单位： | 1.江苏大学药学院 2.植物细胞工程安徽省工程技术研究中心 |
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| 摘 要： | 对水菖蒲内生真菌进行分离，并进行抗菌活性的筛选。采用改良氯化三苯四氮唑显色(TTC)微量稀释法筛选抗菌活性菌株。以18 种常见导致食品腐败的细菌、酵母菌和霉菌为指示菌研究活性菌株的抗菌能力。结果显示，水菖蒲内存在广泛的具有抗菌活性的内生菌，占内生菌总数的40.7%，高活性菌株占内生菌总数的12.39%。其中S9 菌株发酵液具有抗细菌、酵母菌和丝状真菌的广谱抗菌能力，是潜在的食品防腐抗菌资源。
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| 关 键 词： | 水菖蒲 内生菌 抗菌活性 |
| 收稿时间： | 2009-07-13 |
Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L. |
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| ZHOU Xiao-kun,CHEN Jun,HAN Bang-xing,HE Jia,LIU Chang-feng.Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L.[J].Food Science,2010,31(9):211-215. |
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| Authors: | ZHOU Xiao-kun CHEN Jun HAN Bang-xing HE Jia LIU Chang-feng |
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| Affiliation: | 1.College of Pharmacy, Jiangsu University, Zhenjiang 212013, China；2. Anhui Engineering Technology Research Center of Plant
Cell Engineering, Lu’an 237012, China |
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| Abstract: | In order to isolate and screen endophytic fungi with inhibitory activity from Acorus calamus L.,active strains were screened through modified TTC micro-dilution method.MIC(minimum inhibitory concentration) and MBC(minimum bactericidal concentration) were detected using 18 food spoilage strains.Results indicated that 40.7% endophytes exhibited antimicrobial activity and 12.39% endophytes exhibited higher antimicrobial activity.In addition,strain S9 exhibited the broadspectrum antimicrobial activity to bacter... |
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| Keywords: | Acorus calamus L endophytic fungi antibacterial activity |
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