Repeated use of immobilized lipase for monoacylglycerol production by solid-phase glycerolysis of olive oil |
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Authors: | Roxana Rosu Yuki Uozaki Yugo Iwasaki Tsuneo Yamane |
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Affiliation: | (1) Laboratory of Molecular Biotechnology, Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Chikusa-ku, 464 Nagoya, Japan |
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Abstract: | By using immobilized lipase for production of monoacylglycerol (MAG) by solid-phase glycerolysis of fats and oils, the enzyme could be recovered easily from the reaction mixture and recycled to reduce the cost of the catalyst. Several support materials (CaCO3, CaSO4·2H2O, Ca2P2O7, and Celite) were screened for immobilization of Pseudomonas sp. lipase by adsorption and tested for solid-phase glycerolysis of olive oil. Immobilization made the reuse of enzyme feasible. CaCO3 proved to be the best support: 90% MAG (wt% in the glycerolfree reaction mixture after 72 h of reaction time) was obtained until the fifth use, 80% after the seventh use, and 60% after the tenth use. The same support was found suitable for immobilization of two other bacterial lipases from Chromobacterium viscosum and Pseudomonas pseudoalkali. |
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Keywords: | Bacterial lipases CaCO3 CaSO4·2H2O Ca2P2O7 Celite enzyme adsorption glycerolysis immobilized lipase monoacylglycerol reuse of immobilized enzyme |
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