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Effect of addition of Tween 80 and potassium dihydrogenphosphate to basal medium on the isolation of marine eukaryotes, thraustochytrids
Authors:Taoka Yousuke  Nagano Naoki  Okita Yuji  Izumida Hitoshi  Sugimoto Shinichi  Hayashi Masahiro
Affiliation:Department of Biological Production and Environmental Science, Laboratory of Marine Bioscience, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan.
Abstract:Tween 80, KH(2)PO(4) and tomato juice were added to basal medium for the isolation of thraustochytrids. By the addition of Tween 80 and KH(2)PO(4), the number of thraustochytrids isolated from seawater increased. KH(2)PO(4) and Tween 80 were considered to be useful for isolating thraustochytrids.
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