| 具有ACE抑制活性的大豆肽的RP-HPLC分离和结构鉴定 |
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| 引用本文： | 钟芳,张晓梅,麻建国.具有ACE抑制活性的大豆肽的RP-HPLC分离和结构鉴定[J].食品与机械,2006,22(6):8-11,29. |
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| 作者姓名： | 钟芳 张晓梅 麻建国 |
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| 作者单位： | 江南大学食品学院,江苏,无锡,214036 |
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| 摘 要： | 在优化RP-HPLC洗脱条件的基础上，对经大孔吸附树脂DA201-C、凝胶过滤色谱Sephadex G-15分离得到的ACE抑制活性大豆肽组分进行进一步的纯化。在分离得到的11个组分中，峰9的ACE抑制活性最高，在0．2mg／mL．的浓度下，其ACE抑制率达到96．92％。分析型RP—HPLC纯化组分9得到三个组分，其中组分9-Ⅰ具有最高的ACE抑制活性，为98．46％，其含量约占组分9的50％以上。经过LC-MS序列分析，降血压组分9-Ⅰ的分子质量为772．4，有三种可能的结构：VISTGAEP、VLSTGAEP和ANSAGTVGP。
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| 关 键 词： | 大豆蛋白 ACE抑制活性 分离纯化 |
| 收稿时间： | 2006-08-22 |
Isolation and structure identification of ACE inhibition peptides from soy hydrolytes Fraction with RP-HPLC and amino acid sequence identification |
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| ZHONG Fang,ZHANG Xiao-mei,MA Jian-guo.Isolation and structure identification of ACE inhibition peptides from soy hydrolytes Fraction with RP-HPLC and amino acid sequence identification[J].Food and Machinery,2006,22(6):8-11,29. |
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| Authors: | ZHONG Fang ZHANG Xiao-mei MA Jian-guo |
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| Affiliation: | School of Food Science and Technology, Southern Yangtze University, Wuxi, Jiangsu 214036, China |
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| Abstract: | ACE inhibition soy peptides primarily separated with DA201-C macroporous adsorption resin and Sephadex G-15 was further fractionated with RP-HPLC. Under optimized elution condition, 11 fractions were obtained by RP-HPLC, among which fraction 9 showed the highest ACE inhibition activity, at the concentration of 0.2 mg/mL, the ACE inhibition rate was 96.92%. Further purification of this fraction was carried out with analytical RP-HPLC, 3 fractions were identified, with 9-1 fraction being the most active ACE inhibition peptide ( ACE inhibition rate 98.46%). Amino acid sequence of the peptide was identified with RP-HPLC-MS, there were three possible structure of this peptide, that is,VISTGAEP,VLSTGAEP and ANSAGTVGP, the molecular weight of the peptide was 772.4. |
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| Keywords: | Soy protein ACE inhibition Fractionation |
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