| 多粘菌素B中多粘菌素B1的制备方法研究 |
| |
| 引用本文： | 何凤云,薛蒙伟,黄淼,姜涛.多粘菌素B中多粘菌素B1的制备方法研究[J].化工时刊,2011,25(8):20-22. |
| |
| 作者姓名： | 何凤云 薛蒙伟 黄淼 姜涛 |
| |
| 作者单位： | 1. 南京晓庄学院生物化工与环境工程学院,江苏南京,210017
2. 上海张江药谷公共服务平台有限公司,上海,201203 |
| |
| 基金项目： | 上海市科委公共服务平台资助项目(09DZ2292100) |
| |
| 摘 要： | 用反相制备液相色谱从多粘菌素B中分离得到了高纯度多粘菌素B1单体.采用YMC - ODS -A色谱柱,对制备条件进行了优化.最佳制备条件为:流动相组成,含0.1％三氟乙酸的乙腈/水(21/79,V/V)；流速为25 mL/min;单针上样量50 mg;收集波长210 nm;收集信号强度:10 mAU;收集液在40℃下旋...
|
| 关 键 词： | 反相制备液相色谱 多肽类抗生素 多粘菌素B 多粘菌素B1 |
Study on Separation and Purification of Polymyxin B_1 from Polymyxin B |
| |
| He Fengyun,Xue Mengwei,Huang Miao,Jiang Tao.Study on Separation and Purification of Polymyxin B_1 from Polymyxin B[J].Chemical Industry Times,2011,25(8):20-22. |
| |
| Authors: | He Fengyun Xue Mengwei Huang Miao Jiang Tao |
| |
| Affiliation: | He Fengyun Xue Mengwei Huang Miao* Jiang Tao*(Schcool of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Jiangsu Nanjing 210017,*Shanghai Pharma Engine Co.,Ltd,Shanghai 201203) |
| |
| Abstract: | A new RP-HPLC method was established for separation and purification of polymyxin B1 from polymyxin B.Purifiation and analysis were both performed on a YMC-ODS-A column.Experimental parameters were investigated.The optimum separation condition was： mobile phase,acetonitrile-water（21∶79,V/V） containing 0.1% trifluoroacetic acid;flow rate,25 mL/min;sample amount of single,50 mg;collection wavelenth,210 nm;collection signal strength,10 mAU;collection liquid treatment： rotary evaporation at 40℃ and freeze-drying.The purity of the obtained polymyxin B1 product was 98.76%. |
| |
| Keywords: | preparative HPLC polypeptide antibiotics polymyxin B polymyxin B1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
