固定化光合细菌对含酚废水的生物降解试验

对实验室保存的光合细菌用不同浓度的模拟含酚废水进行驯化后,采用RCVBN液体培养基对光合细菌进行扩大培养,并用海藻酸钠-壳聚糖-活性炭微胶囊法对光合细菌进行固定。分别用固定化光合细菌和非固定化光合细菌在不同的温度和不同的接种量下对含酚废水进行降解。以苯酚含量为降解效果指标,结果表明:两种状态下的光合细菌均可以降解含酚废水,但是它们对苯酚的降解率各不相同,固定化光合细菌优于非固定化光合细菌。进一步的正交试验结果表明:影响固定化光合细菌处理含酚废水各因素的影响次序分别为:温度、接种量、pH值,其最佳降解条件是温度为30℃,接种量为20%,pH值为7.0,此时废水中苯酚降解率为85.3%。

Experiments on the biodegradation of phenol wastewater by immobilized photosynthetic bacteria

After domestication with phenol wastewater and culturation in an RCVBN medium, photosynthetic bacteria （PSB） were immobilized by sodium alginate-ehitosan-powder active carbon mierocapsules. The immobilized PSB. and dissociated PSB were both used to biodegrade phenol wastewater at different temperatures and under different inoculation quantities. By measuring the concentration of the phenol, it was found that both forms of PSB have the ability to degrade phenol wastewater, the treatment effect varied under different states, and the effect of immobilized PSB is better. An orthogonal experiment indicates that the most important factor affecting the degradation of phenol wastewater with immobilized PSB is temperature, followed by inoculation quantity and pH. When the temperature is 30℃, the inoculation quantity is 20%, and pH is 7.0, the best degradation effect is obtained and the removal rate reaches 85.3%.