| Cytotoxicity of fullerenols on Tetrahymena pyriformis |
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| 作者姓名： | ZHAO Qun-Fen[ ] ZHU Ying RAN Tie-Cheng LI Jun-Gang LI Qing-Nuan LI Wen-Xin |
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| 作者单位： | [1]Laboratory of Nano, Biology and Medicine, Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800, China [2]Faculty of Life Science and Bioengineering, Ningbo University, Ningbo 315211, China |
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| 基金项目： | 浙江省自然科学基金
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国家重点基础研究发展计划(973计划) |
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| 摘 要： | With the increasing use of fullerenes and their derivatives in a variety of fields, the toxicity and effects of fullerenes on humans and the environment have received considerable attention. In this study, the cytotoxicity of fullerene derivative, C60（OH）x, on Tetrahymena pyriformis was investigated. Cell growth inhibition was evaluated by counting with an optical microscope, and the generation time was calculated. It was indicated that the fullerenols caused a dose-dependent growth inhibition of the cells. The morphologic change in the damaged macronucleus of cells was observed using a fluorescent microscope. Superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX）, and glutathione reductase （GR） levels were also measured for Tetrahymena pyriformis, using conventional methods. The results showed that fullerenols could reduce GSH-PX and GR activities. But no noticeable difference in SOD activity was observed between the treated groups and the control group. This indicated that the antiproliferative effect of fullerenols might be mediated by the reduction in the activities of GSH-PX and GR of cells and the destruction of the macronucleus.
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| 关 键 词： | 细胞毒性 四膜虫属梨状肌 富勒醇 过氧化物歧化酶 谷胱甘肽过氧化酶 |
| 收稿时间： | 03 8 2006 12:00AM |
Cytotoxicity of fullerenols on Tetrahymena pyriformis |
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| ZHAO Qun-Fen, ZHU Ying RAN Tie-Cheng LI Jun-Gang LI Qing-Nuan LI Wen-Xin.Cytotoxicity of fullerenols on Tetrahymena pyriformis[J].Nuclear Science and Techniques,2006,17(5):280-284. |
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| Authors: | ZHAO Qun-Fen ZHU Ying RAN Tie-Cheng LI Jun-Gang LI Qing-Nuan LI We-Xin |
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| Affiliation: | 1. Laboratory of Nano, Biology and Medicine, Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800, China;Faculty of Life Science and Bioengineering, Ningbo University, Ningbo 315211, China
2. Laboratory of Nano, Biology and Medicine, Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800, China |
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| Abstract: | With the increasing use of fullerenes and their derivatives in a variety of fields, the toxicity and effects of fullerenes on humans and the environment have received considerable attention. In this study, the cytotoxicity of fullerene derivative, C60(OH)x, on Tetrahymena pyriformis was investigated. Cell growth inhibition was evaluated by counting with an optical microscope, and the generation time was calculated. It was indicated that the fullerenols caused a dose-dependent growth inhibition of the cells. The morphologic change in the damaged macronucleus of cells was observed using a fluorescent microscope. Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX),and glutathione reductase (GR) levels were also measured for Tetrahymena pyriformis, using conventional methods.The results showed that fullerenols could reduce GSH-PX and GR activities. But no noticeable difference in SOD activity was observed between the treated groups and the control group. This indicated that the antiproliferative effect of fullerenols might be mediated by the reduction in the activities of GSH-PX and GR of cells and the destruction of the macronucleus. |
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| Keywords: | Fullerenol Cytotoxity Tetrahymena pyriformis |
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