Inoculation of Filamentous Fungi in Manufactured Gas Plant Site Soils and PAH Transformation |
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| Authors: | Rachel Rama Jean-Claude Sigoillot Véronique Chaplain Marcel Asther Claude Jolivalt Christian Mougin |
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| Affiliation: | 1. KREBS SA , 1 Rue des Hérons, 78180, Montigny le Bretonneux, France;2. Aventis Crop Science , 14-20 Rue P. Baizet, BP 9163, 69263, Lyon Cedex, 09, France;3. Institut National de la Recherche Agronomique (INRA), Unité de Biotechnologie des Champignons Filamenteux, Faculté des Sciences de Luminy, Centre d'Enseignement Supérieur en Biotechnologies/ESIL, Parc Scientifique et Technologique , CP 925, 163 Avenue de Luminy, 13288, Marseille Cedex 09, France;4. Institut National de la Recherche Agronomique (INRA) , Unité de Phytopharmacie et Médiateurs Chimiques , Route de Saint-Cyr, 78026, Versailles Cedex, France |
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| Abstract: | Solid carriers have been developed to inoculate Trametes versicolor and Cunninghamella elegans into manufactured gas plant site soils. Pelleted wheat bran carriers were very efficient in stimulating the growth of fungi in an industrial soil containing about 2800 mg kg?1 PAHs. Fungal biomass and activity of extracellular laccases, enzymes produced by T. versicolor as markers of metabolic activity in the contaminated soil, both decreased after 2 weeks of incubation. Supplementing the soil with a mixture of carbon, nitrogen and phosphorus enhanced the fungal activity period. A 38% decrease of solvent extractable PAHs was observed in manufactured gas plant site soils when supplemented with T. versicolor, Glucidex 19TM, ammonium nitrate, lime phosphate and Montanox 80TM, after 20 weeks. Then, the degradation proceeded more slowly during the following 30 weeks, and reached 43% of initial extractable PAHs. Some factors governing a limited PAH biotransformation in the soil are discussed. |
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| Keywords: | Polycyclic aromatic hydrocarbons Bioremediation Immobilization Microcosms Manufactured gas plants Fungus |
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