Metabolization of the Polycyclic Aromatic Hydrocarbon Benzo( A )Pyrene by a Non-White Rot Fungus ( Fusarium Solani ) in a Batch Reactor |
| |
Authors: | E. Veignie C. Rafin P. Woisel A. Lounes-Hadj Sahraoui F. Cazier |
| |
Affiliation: | 1. Université du Littoral C?te d'Opale , Calais, France;2. Laboratoire de Synthèse Organique et Environnement , Université du Littoral C?te d'Opale , Dunkerque, France;3. Laboratoire de Mycologie-Phytopathologie-Environnement , Université du Littoral C?te d'Opale , Calais, France;4. Centre Commun de Mesure , Université du Littoral C?te d'Opale , Dunkerque, France |
| |
Abstract: | To investigate metabolization of benzo[ a ]pyrene (B a P), a high-molecular-weight polycyclic aromatic hydrocarbon consisting of five aromatic rings, by a Deuteromycete Fusarium solani , a culture with [7,10- 14 C]benzo[ a ]pyrene was carried out in a batch fermentor under cometabolic conditions. In spite of drastic culture conditions with a high load of B a P (302 mg in a 1.6 liters), analysis of the growth and substrate depletion kinetics showed a classical pattern. The evolution of 14 CO 2 release demonstrated that B a P mineralization by the non-white rot fungus F. solani occurred rapidly at early stages of fermentation (15 hr) during the germination process. At the end of fermentation, 1.2% of the B a P was evolved as 14 CO 2 and 5.3% was incorporated in biomass. B a P metabolization was also confirmed by isolating metabolic products, extracted from mycelia and identified as 1,6-benzo[ a ]pyrene quinone and 3,6-benzo[ a ]pyrene quinone, by mass and ultraviolet (UV) spectroscopic analyses. |
| |
Keywords: | Benzo[a]pyrene Fusarium Solani Mineralization |
|
|