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Relative Genotoxicities of PAH of Molecular Weight 252 AMU in Coal Tar-Contaminated Sediment |
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| Authors: | C. H. Marvin S. Mehta D. Lin B. E. McCarry D. W. Bryant |
| Affiliation: | 1. Aquatic Ecosystem Restoration Branch, National Water Research, Institute, Environment Canada , 867 Lakeshore Road, Burlington, Ontario, Canada;2. Department of Chemistry , McMaster University , 1280 Main Street West, Hamilton, Ontario, Canada , L8S 4M1;3. Department of Biochemistry , McMaster University , 1280 Main Street West, Hamilton, Ontario, Canada , L8S 4M1 |
| Abstract: | Bioassay-directed chemical fractionation methodology was used to calculate relative mutagenic potencies of polycyclic aromatic hydrocarbons (PAH) of molecular weight 252 amu in coal tarcontaminated sediment from Sydney Harbour, Nova Scotia. A normal phase HPLC technique was used to separate organic solvent extracts into fractions containing isomeric PAH of a single benzologue class. Bioassays with Salmonella typhimurium strain YG1025 with the addition of oxidative metabolism (S9) showed that approximately 50% of the mutagenic activity observed in the sediment extract was associated with PAH of molecular weight 252 amu. Further separation of the 252 PAH fraction using reversed phase HPLC yielded subfractions containing individual compounds; bioassay dose-response curves for these subfractions showed that benzo[a]pyrene was responsible for approximately 75% of the activity of the 252 PAH fraction. |
| Keywords: | polycyclic aromatic hydrocarbons Salmonella typhimurium bioassay-directed fractionation genotoxicity coal tar sediments |