Direct Synthesis and Characterization of Site-Specific Deoxyguanosyl and Deoxyadenosyl Adducts Derived from the Binding of Pah Diol Epoxides to Oligonucleotides
1. American Health Foundation , Valhalla, NY, 10595;2. Chemistry Department , New York University , New York, NY, 10003;3. Lawrence Livermore National Laboratory , Livermore, CA, 94551
Abstract:
The direct synthesis method is a relatively simple approach for generating modified oligonucleotides of defined base sequence with site-specifically placed polycyclic aromatic hydrocarbon diol epoxide-modified deoxyguanosyl and deoxyadenosyl residues. Up to 5–10 mg quantities of several different modified oligonucleotides have been synthesized for detailed NMR structural studies. Smaller amounts of modified oligonucleotides containing a single modified guanosyl residue in oligonucleotides 10–11 bases long containing up to three other unmodified guanine residues can be readily generated in smaller quantities for site-directed mutagenesis and other studies.