Molecular dynamics simulations of the N-linked oligosaccharide of the lectin from Erythrina corallodendron |
| |
Authors: | Naidoo KJ; Denysyk D; Brady JW |
| |
Affiliation: | Department of Food Science, Cornell University, Ithaca, NY 14850, USA. |
| |
Abstract: | Molecular dynamics simulations have been used to model the flexibility of
the seven-sugar oligosaccharide of the lectin from Erythrina corallodendron
in three separate simulations: one of the isolated oligosaccharide in
vacuo, one of the oligosaccharide in solution and one of the
oligosaccharide linked to the protein in solution. Adiabatic conformational
energy maps were prepared for each of the disaccharide linkages as a means
of interpreting the observed dynamics and conformational averages in terms
of intramolecular energy. The inclusion of aqueous solvent molecules
appears to be necessary to reproduce the experimental conformational
behavior, which also cannot be predicted well from conformational energy
maps for the disaccharide linkages alone. The crystallographically
determined conformation does not appear to be induced by the crystal
dimerization, but is rather stable in solution. The build-up of
fluctuations along the successive linkages of the oligosaccharide is
significant and would be sufficient to prevent branch residues from being
located in most crystal structure determinations. Good general agreement
between the calculated solution structure and the average structure
determined by NMR was found for most of the oligosaccharide linkages.
|
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|