| 外源DNA通过花粉管通道法导入玉米的研究 |
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| 引用本文： | 刘丹梅.外源DNA通过花粉管通道法导入玉米的研究[J].辽东学院学报(自然科学版),2004,11(4):5-6,8. |
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| 作者姓名： | 刘丹梅 |
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| 作者单位： | 辽东学院生物系,辽宁,丹东,118003 |
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| 摘 要： | 利用花粉管通道技术,以抗虫的胰蛋白酶抑制剂基因(CpTI)为供体,以8个品种玉米为受体,进行外源DNA直接导入,获得了大量的转基因植株,通过对抗性植株的初步分子鉴定,证实外源CpTI基因存在并整合入玉米基因组中。
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| 关 键 词： | 玉米 花粉管通道 外源DNA |
| 文章编号： | 1008-2174(2004)04-0005-03 |
| 修稿时间： | 2004年10月14 |
Introduction of Exogenous DNA into Maize via Pollen Tube Pathway |
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| LIU Dan-mei.Introduction of Exogenous DNA into Maize via Pollen Tube Pathway[J].Journal of Liaodong University：Natural Sciences,2004,11(4):5-6,8. |
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| Authors: | LIU Dan-mei |
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| Abstract: | Exogenous DNA has transformed CpTI into maize by pollen tube pathway, and off springs have been obtained. These plants were carried on further screen of kanamycin and molecular test. Some of them were confirmed to be transgenic plants by assays of PCR. The molecular test proved that the foreign gene was inherited and expressed in maize plant. |
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| Keywords: | Maize pollen tube pathway exogenous DNA |
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