自动化微生物比浊法测定血清中环丙沙星浓度

目的 建立自动化微生物比浊法测定血清环丙沙星(CPLX)浓度。方法 以标准血清配制系列浓度CPLX 与10⁷ cfu/ml 标准菌ATCC 27853 反应, 在自动化微生物分析仪中测定其吸收度。结果 在8 ~ 0.0625 μg /ml 浓度范围内, 浓度与吸收度呈线性关系(r =0.9950, n =8), 回归方程为C =9.4020 -22.55 A, 最低检测浓度为0.03 μg /ml 。方法回归率为96.5 % ~ 108.0 %, 日内变异系数为2.11% ~ 9.13 %, 日间变异系数为1.99 % ~ 9.72 %。结论 该方法为一快速、方便、精确的测定CPLX 的方法。

Microbiological turbidimetric method in determination of serum concentrations of ciprofloxaxin

Aim The application of microbiological turbidimetric method to determination of serum concentrations (C) of ciprofloxacin (CPLX) was studied. Methods A series diluted concentrations of CPLX were prepared with blank serum. The solution was put in a research unit with standard bacteria (ATCC 27853) dilution of 10⁷ cfu/ ml and incubated in Avantage analysis system. Absorbance (A) was recorded once every 5 min in a wavelength of 670 nm. Results There was a liner relationship (r =0.9950, n =8) between concentrations of CP LX and absorbance. The regression equation was C =9.4020-22.55 A. The lowest detectable concentration was 0.03 μg /ml. The rate of recovery of the method was 96.5 % ~ 108.0 %. The coefficient of variation within a day was 2.11% ~ 9.13 % while the coefficient of variation in different days was 1.99 % ~ 9.72 %. Conclusion The microbiological turbidimetry is a rapid, convenient and accurate method to determine the concentration of CPLX.