纳洛酮对人离体肾脏缺血再灌注损伤的影响

目的 研究纳洛酮对人离体肾脏缺血再灌注损伤的影响。方法 用人离体肾脏, 实验组在灌注液中加入纳洛酮(NAL)0.8μg/ml, 分别在0 ~ 10 min 、10 ~ 20 min 和20 ~ 30 min 时收集灌注流出液, 测定其丙二醛(MDA)、过氧化物歧化酶(SOD)及肾组织匀浆中的Na⁺, K⁺-ATP 酶、Ca²⁺-AT P 酶含量, 并做病理观察。结果 上述各项指标在用NAL 组与对照组比较均无显著性差异。结论 NAL 对离体肾脏缺血再灌注无明显影响, NAL 对在体肾脏缺血再灌注的保护作用不是直接作用在肾脏本身。

Effects of naloxone on human renal postischemic reperfusion in vitro

Aim The effect of naloxone (NAL) on human renal postischemic reperfusion in vitro was observed. Methods Six human kidneys in two groups were reperfused separately by either normal perfusion solution or the solution added by 0.8 μg/ml of naloxone. Malondialdehyde (MDA) and superoxide dismutase (SOD) in the reperfusion solution and Na⁺, K⁺-ATP ase and Ca²⁺-ATPase in the renal tissue were determined. The histopathological structure of kidneys was obseved. Results All the above parameters in the treatment had no significant difference from those in the control group. Conclusion NAL had no obvious effect on human renal postischemic reperfusion in vitro. It was supposed that though NAL had protective effect on renal postischemic reperfusion in vivo, it did not directly affect the kidney.