乳酸菌微生物燃料电池产电性能及产电机理研究

采用双室微生物燃料电池（MFC），以乳酸菌为产电微生物，并以葡萄糖为唯一的电子供体，研究MFC的产电性能以及乳酸菌MFC产电机理。在30 ℃下，底物浓度为1.5 g/L时，该MFC的开路电压稳定在500 mV。实验条件下测得该MFC的最大功率密度为393.23 mW/m²，内阻约为500 Ω。利用气相色谱分析乳酸菌MFC产电过程中代谢产物的含量变化，实验数据表明无论是不参与产电的正常代谢途径还是产电过程中，都涉及到乳酸菌的同型乳酸发酵途径、异型乳酸发酵的经典途径和双歧杆菌发酵途径。在乳酸菌MFC运行过程中人为添加乙醇，该实验结果显示乙醇不利于乳酸菌产电，表明乳酸菌的异型乳酸发酵途径是乳酸菌进行产电的关键代谢途径。

Property and Mechanism of Electricity Production on Lactic Acid Bacteria Microbial Fuel Cells

The dual-chamber microbial fuel cell （MFC） used lactic acid bacteria for the electricity production microorganisms and adopted glucose as the only electron donor. The proPerty and mechanism of electricity production were also investigated on lactic acid bacteria microbial fuel cells. When the concentration of the substrate was 1.5 g/L at 30 ℃, the open-circuit voltage of the MFC kept at 500 mV. Under the experimental conditions, the maximum power density reached 393.23 mW/m2 and the internal resistance was about 500 Ωon the MFC. The change of the metabolites contents in the lactic acid bacteria MFC electricity production process was analyzed by the gas chromatography. The results indicated that both the normal metabolic pathways which was not involved in the production of electricity and the electricityproduction process, were related to homolactic fermention, the classical pathway of heterolactic fermentation and bifidobacterium fermentation. After adding ethanol in the process of the electricity production for lactic acid bacteria MFC, the results showed that ethanol was not conducive to the electricity production. This indicated that the key metabolic pathway in lactic acid bacteria producing electricity process was heterolactic fermentation.