| 一氧化氮合成酶对AQ4N放射增敏作用的影响研究 |
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| 引用本文： | 孔肇路,金一尊,Chinje E C,Stratford I J.一氧化氮合成酶对AQ4N放射增敏作用的影响研究[J].辐射研究与辐射工艺学报,2006,24(3):159-162. |
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| 作者姓名： | 孔肇路 金一尊 Chinje E C Stratford I J |
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| 作者单位： | 1. 复旦大学放射医学研究所,上海,200032
2. 英国曼彻斯特药学院,英国,M13 9PL |
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| 基金项目： | 国家自然科学基金;国家科技攻关项目 |
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| 摘 要： | 评价1，4-二-2-（二甲氨基-N-氧化物）乙醇]氨基]5，8-双羟基氧化蒽-9，10-二酮（（1，4-bis{2-（dimethylamino-N-oxide）ethyl]amino}-5，8-dihydroxyanthracene-9，10dione），AQ4N）辐射增敏效果和乏氧选择性，分析一氧化氮合成酶（Inducible nitlogen monoxide synthase，iNOS）对AQ4N代谢活化的影响和作用特点。结果显示AQ4N是一种低毒性的化合物，仅在乏氧条件显示较弱的细胞毒性，可以引起肿瘤细胞凋亡。AQ4N对肿瘤细胞尤其是乏氧肿瘤细胞具有良好的辐射增敏作用。同亲本细胞相比，iNOS基因转染的细胞克隆，仅在乏氧条件下显示出对AQ4N化学敏感性的增强和细胞的G2／M期阻滞。提示iNOS仅在乏氧条件下参与AO4N的代谢活化过程。
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| 关 键 词： | 生物还原 细胞凋亡 p450还原酶 辐射增敏 |
| 收稿时间： | 2005-12-09 |
| 修稿时间： | 2006-02-13 |
Effects of iNOS on radiosensitization of AQ4N in vitro |
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| KONG Zhaolu,JIN Yizun,Chinje E C,Stratford I J.Effects of iNOS on radiosensitization of AQ4N in vitro[J].Journal of Radiation Research and Radiation Processing,2006,24(3):159-162. |
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| Authors: | KONG Zhaolu JIN Yizun Chinje E C Stratford I J |
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| Affiliation: | 1 The institute of radiation medicine, Fudan university, Shanghai 200032; 2 .Ontology Lab, the Pharmacy School of Manchest University, UK, M13 9PL |
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| Abstract: | The study aims to evaluate radiosensitization of AQ4N, a new bioreductive agent, and to clarify the role of iNOS in its metabolism. It seemed that AQ4N was a hypoxia-selective radiosensitizer and mainly caused ceils apotosis, iNOS-gene transfected cells showed G2/M phase block 24 hours after treated with AQ4N under hypoxia. The study showed that iNOS could be a target enzyme taking part in AQ4N bioreduction in anaerobic condition. Further work should be done to clarify the enzymology involved in AQ4N bioactivation to improve the application of AQ4N as a radiosensitizer in clinic. |
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| Keywords: | AQ4N |
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