Synthesis of polymer materials for use as cell culture substrates |
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Authors: | Sophie Lakard,Nadè ge Morrand-Villeneuve,Boris Lakard,Guillaume Herlem,Bernard Fahys |
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Affiliation: | a Laboratoire de Chimie des Matériaux et Interfaces, University of Franche-Comté, IUT, 30 Avenue de l’Observatoire, 25009 Besançon, France b Laboratoire de Neurosciences, University of Franche-Comté, Place Leclerc, 25030 Besançon, France c Laboratoire de Physique de l’Université de Bourgogne, University of Bourgogne, 9 Avenue Savary, 21078 Dijon, France d Laboratoire de Chimie des Matériaux et Interfaces, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France e Laboratoire d’Optique P.M. Duffieux, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France |
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Abstract: | Up to today, several techniques have been used to maintain cells in culture for studying many aspects of cell biology and physiology. More often, cell culture is dependent on proper anchorage of cells to the growth surface. Thus, poly-l-lysine, fibronectin or laminin are the most commonly used substrates. In this study, electrosynthesized biocompatible polymer films are proposed as an alternative to these standard substrates. The electrosynthesized polymers tested were polyethylenimine, polypropylenimine and polypyrrole. Then, the adhesion, proliferation and morphology of rat neuronal cell lines were investigated on these polymer substrates in an attempt to develop new and efficient polymer materials for cell culture. During their growth on the polymers, the evolution of the cell morphology was monitored using both confocal microscopy and immunohistochemistry, leading to the conclusion of a normal development. An estimation of the adhesion and proliferation rates of rat neuronal cell cultures indicated that polyethylenimine and polypropylenimine were the best substrates for culturing olfactory neuronal cells. A method to favour the differentiation of the neuronal cells was also developed since the final aim of this work is to develop a biosensor for odour detection using differentiated neuronal cells as transducers. Consequently, a biosensor was microfabricated using silicon technology. This microsystem allowed us to culture the cells on a silicon wafer and to position the cells on certain parts of the silicon wafer. |
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Keywords: | Electrochemistry Polymers Cell culture Atomic force microscopy Microsystem Neurosciences |
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