| 培养基成分对冬虫夏草菌生长的影响及其优化 |
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| 引用本文： | 杜晓蒙,孙海龙,崔建东. 培养基成分对冬虫夏草菌生长的影响及其优化[J]. 中国酿造, 2011, 0(3) |
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| 作者姓名： | 杜晓蒙 孙海龙 崔建东 |
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| 作者单位： | 河北科技大学,生物科学与工程学院,河北,石家庄,050018 |
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| 基金项目： | 河北省高等学校科学研究青年基金，2010河北科技大学大学生科技创新基金项目 |
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| 摘 要： | 以冬虫夏草菌体量为指标,研究了培养基中不同成分对虫草生长的影响,利用Plackett-Burman(PB)试验设计筛选出影响虫草菌生长的关键培养基成分,然后利用最陡爬坡试验和中心组合设计试验优化和确立最佳培养基配方,结果表明,葡萄糖、酵母粉和KH2PO4对虫草的生长有显著影响,优化后的培养基成分为葡萄糖59.35g/L、KH2PO4 1.47g/L、酵母浸粉16.83g/L、蛋白胨10g/L、MgSO4·7H2O0.1g/L.利用此培养基获得的虫草菌体量为26.86g/L,是优化前的2.5倍,为虫草工业化生产提供参考.
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| 关 键 词： | 冬虫夏草 培养基 菌体量 统计学优化 |
Effects of medium components on growth of mycelium of Cordyceps militaris and medium optimization |
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| DU Xiaomeng,SUN Hailong,CUI Jiandong. Effects of medium components on growth of mycelium of Cordyceps militaris and medium optimization[J]. China Brewing, 2011, 0(3) |
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| Authors: | DU Xiaomeng SUN Hailong CUI Jiandong |
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| Affiliation: | DU Xiaomeng,SUN Hailong,CUI Jiandong~* (College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050018,China) |
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| Abstract: | Using mycelium biomass as evaluation parameter,effects of medium components on growth of Cordyceps militans were investigated.The critical components were screened with Plackett-Buman experimental design and the optimum composition of medium was determined with the steepest ascent method and Center Composite Design(CCD).The results showed that glucose,KH_2PO_4 and yeast extract had the most significant effects on mycelium biomass.The optimum medium for growth of mycelium were as follows,59.35g/L glucose,16.... |
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| Keywords: | Cordyceps militaris medium mycelium biomass statistic-based experimental design |
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