| 海洋放线菌的筛选和抑菌活性的发酵条件优化 |
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| 引用本文： | 唐婧媛,陈莉,薛永常. 海洋放线菌的筛选和抑菌活性的发酵条件优化[J]. 中国酿造, 2011, 0(2) |
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| 作者姓名： | 唐婧媛 陈莉 薛永常 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁,大连,116034 |
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| 摘 要： | 从大连海域筛出33株放线菌,其中4株具有显著地抑菌活性,放线菌D09的抑菌活性最高,并具有广谱抗菌性.以金黄色葡萄球菌为指示菌,对放线菌D09发酵条件和理化性质进行了研究,正交试验表明,其最优发酵条件为:菌龄为48h,接种量10.0%6,发酵培养基初始pH值为7,培养条件为25℃,装样量为10%,发酵培养基配方为可溶性淀粉15g/L,KNO3 1g/L,K2HPO4·H2O0.25g/L,MgS04·7H2O0.25g/L.在最优条件下,放线菌D09生长最快,抑菌能力最强.
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| 关 键 词： | 海洋放线菌 抗菌活性物质 发酵 |
Isolation and fermentation optimization of marine actinomycetes with antimicrobial activity |
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| TANG Jingyuan,CHEN Li,XUE Yongchang. Isolation and fermentation optimization of marine actinomycetes with antimicrobial activity[J]. China Brewing, 2011, 0(2) |
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| Authors: | TANG Jingyuan CHEN Li XUE Yongchang |
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| Affiliation: | TANG Jingyuan,CHEN Li,XUE Yongchang(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | 33 marine actinomycetes were isolated from marine mud samples collected from different Dalian sea areas.And 4 of the isolated marine actinomycetes had antimicrobial activities.The strain named D09 had highest antimicrobial activity and a broad antimicrobial spectrum.Using Staphylococcus aureus as a indicator,the fermentation conditions and physicochemical properties of D09 were studied.The optimum fermentation conditions for the production of antimicrobial compounds were determined by orthogonal tests as fo... |
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| Keywords: | marine actinomycete antimicrobial compounds fermentation |
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