| Bacillus licheniformis产凝乳酶培养基的优化 |
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| 引用本文： | 张卫兵,宋曦,贺晓玲,甘伯中.Bacillus licheniformis产凝乳酶培养基的优化[J].中国酿造,2011(2). |
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| 作者姓名： | 张卫兵 宋曦 贺晓玲 甘伯中 |
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| 作者单位： | 甘肃农业大学食品科学与工程学院甘肃省干酪素工程技术研究中心,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省农业生物技术专项项目，甘肃省科技重大专项项目 |
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| 摘 要： | 以Bacillus lichcniformis为研究对象,通过单因素试验结合正交设计对其产凝乳酶培养基成分进行了优化,得到培养基成分最优组合为麸皮汁浓度16%,葡糖糖浓度为7%,酪蛋白添加量为5%,氮源添加量为5%,优化后凝乳酶活力可达134.72SU/mL,比原来提高39.93%.
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| 关 键 词： | 地衣芽孢杆菌 凝乳酶 凝乳酶活力 |
Optimization of culture media of Bacillus licheniformis with high yield of chymosin |
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| ZHANG Weibing,SONG Xi,HE Xiaoling,GAN Bozhong.Optimization of culture media of Bacillus licheniformis with high yield of chymosin[J].China Brewing,2011(2). |
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| Authors: | ZHANG Weibing SONG Xi HE Xiaoling GAN Bozhong |
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| Affiliation: | ZHANG Weibing,SONG Xi,HE Xiaoling,GAN Bozhong(College of Food Science and Engineering,Gansu Casein Engineering Technical Research Centre,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | The medium components for chymosin production with Bacillus licheniformis were optimized with single factor test and orthogonal experimental design.The optimum media components were as follows: extracts of wheat bran 16%,glucose 7%,casein 5%,and nitrogen source 5% and activity of chymosin could reach 1347.19 SU/ml with the optimum medium,which increased by 39.93%. |
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| Keywords: | Bacillus licheniformis chymosin chymosin activity |
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