超高效液相色谱法同时测定食品塑料包装材料中紫外吸收剂和抗氧化剂的迁移量

目的建立食品塑料包装材料中限用紫外吸收剂(UV-0、UV-9、UV-71、UV-326、UV-327、UV-234)和抗氧化剂(2246、425、TH-1790、3114和1076)迁移量的超高效液相色谱(ultra performance liquid chromatography,UPLC)测定方法。方法优化仪器条件及模拟液萃取条件,考察超纯水、3%乙酸水(V:V)溶液、10%乙醇水(V:V)溶液及95%乙醇水(V:V)溶液等4种食品模拟物中紫外吸收剂和抗氧化剂的迁移量,并将建立的方法用于市售样品迁移量的检测。结果 11种目标物质在相应的浓度范围内线性关系良好,相关系数r~2大于0.9992,方法检出限为0.5~2.0 mg/L;平均加标回收率为81.2%~113.2%,相对标准偏差(n=6)为2.8%~8.5%。结论该检测方法灵敏、准确,满足欧盟指令(EU)No.10/2011和GB 9685-2008对食品塑料接触材料及制品中紫外吸收剂和抗氧化剂特定迁移量(SML)的限量要求,适用于食品塑料包装材料中紫外吸收剂和抗氧化剂的迁移量的测定。

Simultaneous determination of migration amounts of the ultraviolet absorbers and antioxidants by ultra performance liquid chromatography in plastic food packaging materials

Objective To establish a method based on migration tests using food simulants for the determination of migration levels of the ultraviolet absorbers (V-0, UV-9, UV-71, UV-326, UV-327, UV-234) and antioxidants (2246, 425, TH-1790, 3114, 1076) in plastic food packaging materials by ultra performance liquid chromatography (UPLC). Methods The instrument conditions and soaking liquid extraction conditions were optimized. According to GB 9685-2008, 4 kinds of food stimulants, including ultrapure water, 3% acetic acid (V:V) solution, 10% ethanol aqueous (V:V) solution and 95% ethanol aqueous (V:V) solution, were investigated. The developed method was applied to detect the migration amount of analytes in different commercial food plastic packages. Results The 11 kinds of analytes presented favorable linearity in the respective linear range, with correlation coefficient (r2) more than 0.9992, and the method detection limits were 0.5~2.0 mg/L. The average recoveries (n=6) ranged from 81.2%~113.2%, and the relative standard deviations (RSD, n=6) were 2.8%~8.5%. Conclusion The method is sensitive, accurate, and can meet the requirements of European Commission Regulation (EU) No 10/2011 and GB 9685-2008 for the specific migration limits (SML) of the ultraviolet absorbers and antioxidants. The method can be adapted to determine the migration of ultraviolet absorbers and antioxidants in plastic food packaging materials.