| 伏马毒素B1新型荧光标记物制备及其在荧光偏振分析技术中的应用 |
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| 引用本文： | 刘继红,Chris Maragos,王 敏,尹海燕,刘冬梅,张军锋,王红旗.伏马毒素B1新型荧光标记物制备及其在荧光偏振分析技术中的应用[J].食品安全质量检测技术,2015,6(12):4810-4815. |
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| 作者姓名： | 刘继红 Chris Maragos 王 敏 尹海燕 刘冬梅 张军锋 王红旗 |
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| 作者单位： | 河南省农业科学院农业质量标准与检测技术研究所,美国农业部USDA国家农业应用研究中心NCAUR食源性病原菌与真菌毒素研究分部,皮奥里亚,河南省农业科学院农业质量标准与检测技术研究所,河南省农业科学院农业质量标准与检测技术研究所,河南省农业科学院农业质量标准与检测技术研究所,河南省农业科学院农业质量标准与检测技术研究所,河南省农业科学院农业质量标准与检测技术研究所 |
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| 基金项目： | 农业部“引进国际先进农业科学技术”重点项目(2011-G5),粮油作物产品未知危害因子识别与已知危害因子安全性评估(GJFP2015007),河南省农业科学院优秀青年科技基金（2013YQ24） |
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| 摘 要： | 目的针对玉米中常见真菌毒素——伏马毒素B_1(FB1)开发新型荧光标记物,从而替代昂贵的6-(4,6-二氯三嗪基)氨基荧光素(6-DTAF)标记物,并在此基础上开发基于荧光偏振原理的伏马毒素快速检测技术。方法采用一锅煮的合成方法,以5-(4,6-二氯三嗪基)氨基荧光素(5-DTAF)为荧光基团制备伏马毒素荧光标记物,然后利用荧光偏振的原理建立基于5-DTAF的伏马毒素快速检测技术。结果基于5-DTAF的FB1荧光标记物与基于6-DTAF的FB1荧光标记物的荧光偏振分析方法具有相同的分析性能,IC_(50)值基本相等。结论采用5-DTAF成功制备了FB1荧光标记物,同时利用该荧光标记物开发了伏马毒素荧光偏振快速检测技术。该分析技术与基于6-DTAF荧光标记物的FB1荧光偏振速测技术相比,具有同样的分析性能,但分析成本降低了一半。
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| 关 键 词： | 伏马毒素B1 氨基荧光素 荧光标记物 荧光偏振 |
| 收稿时间： | 2015/10/12 0:00:00 |
| 修稿时间： | 2015/12/9 0:00:00 |
Preparation and application of new fluorescein-labeled fumonisins B1 in flu-orescence polarization analysis technique |
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| LIU Ji-Hong,Chris Maragos,WANG Min,YIN Hai-Yan,LIU Dong-Mei,ZHANG Jun-Feng and WANG Hong-Qi.Preparation and application of new fluorescein-labeled fumonisins B1 in flu-orescence polarization analysis technique[J].Food Safety and Quality Detection Technology,2015,6(12):4810-4815. |
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| Authors: | LIU Ji-Hong Chris Maragos WANG Min YIN Hai-Yan LIU Dong-Mei ZHANG Jun-Feng and WANG Hong-Qi |
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| Affiliation: | Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Science,Bacterial Foodborne Pathogens and Mycology Research Unit,Peoria,IL ,USA,Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Science,Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Science,Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Science,Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Science,Institute of Quality Standard and Testing Technology for Agro-products,Henan Academy of Agricultural Science |
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| Abstract: | Objective Preparation of new fluorescent tracer against common mycotoxins such as fumonisins B1, to replace expensive 6-(4,6-Dichlorotriazinyl) aminofluorescein (6-DTAF) marker, and development of technique for fumonisins B1 quick detection based on the principle of fluorescence polarization. Methods Using the one-pot method to synthesize 5-(4,6-Dichlorotriazinyl) aminofluorescein (5-DTAF) fluorescent marker, and then adopting fluorescence polarization technique based on 5-DTAF to detect fumonisins B1. Results Using 5-DTAF we synthesized new fluorescent marker of fumonisins B1, and developed fluorescence polarization technology for fumonisins B1 quick detection. Fluorescence polarization technology based on 5-DTAF and 6-DTAF fluorescent marker of fumonisins B1 has almost the same amplitude and IC50 value. However, the price of 5-DTAF is only half of 6-DTAF, so analysis cost is greatly reduced. |
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| Keywords: | fumonisins B1 aminofluorescein fluorescent tracer fluorescence polarization |
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