| Elizabethkingia sp. DBP-WUST对邻苯二甲酸二丁酯的降解特性研究 |
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| 引用本文： | 颜家保,王巧凤,魏鑫,游海. Elizabethkingia sp. DBP-WUST对邻苯二甲酸二丁酯的降解特性研究[J]. 徐州工程学院学报, 2012, 0(3): 16-21 |
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| 作者姓名： | 颜家保 王巧凤 魏鑫 游海 |
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| 作者单位： | 武汉科技大学化学工程与技术学院,武汉430081 |
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| 基金项目： | 湖北省自然科学基金重点基金项目（2011CDA054）; 湖北省教育厅产学研重点项目（CXY2009B002）; 武汉市供需对接项目（200951624497） |
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| 摘 要： | 从武汉石化活性污泥中分离得到1株能高效降解邻苯二甲酸二丁酯（DBP）的菌株DBP-WUST.经形态学观察、生理生化特性试验及16SrDNA鉴定结果表明,该菌为Elizabethkingia sp..研究发现,该菌株较优的生长条件为：温度35℃,pH7,摇床转速150r/min,接种量7%.在较优生长条件下,菌株DBP-WUST能在60h将400mg/L的DBP全部降解.将菌株投加到实际炼油废水中,能有效降低废水的COD.
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| 关 键 词： | DBP 优势菌 筛选 降解特性 Elizabethkingia sp. |
Biodegradation Characteristics of DBP by Elizabethkingia sp. DBP-WUST |
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| YAN Jia-bao,WANG Qiao-feng,WEI Xin,YOU Hai. Biodegradation Characteristics of DBP by Elizabethkingia sp. DBP-WUST[J]. Journal of Xuzhou Istitute of Technology, 2012, 0(3): 16-21 |
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| Authors: | YAN Jia-bao WANG Qiao-feng WEI Xin YOU Hai |
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| Affiliation: | (College of Chemical Engineering and Technology, Wuhan University of Science and Technology, Wuhan 430081, China) |
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| Abstract: | A strain （DBP-WUST）, which could highly utilize DBP as the sole carbon and energy source, was isolated from the active sludge in the refinery wastewater treatment plant of Sinopec Wuhan Petroleum Group Co. Ltd. According to the morphological observation, physiological and biochemical tests, and sequence analysis of 16S rDNA, the strain was identified as Elizabethkingia sp. The optimal temperature, initial pH, shaker rotary speed, and saturation inoculation dosage for strain DBP-WUST uti- lizing DBP was 35 ℃, pH7, 150 r/min and 7%, respectively. The experiments of DBP degradation by the pure culture showed that DBP of 400 mg/L could be degraded completely in 60 h under the optimal growth conditions. Furthermore, the COD of the waste-water could excellently be removed after the strain was added to the actual refinery waste-water. |
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| Keywords: | DBP dominant bacterial isolation degradation characteristics Elizabethkingia sp. |
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