| 中药丹皮残渣固态发酵产酶与糖化工艺的研究 |
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| 引用本文： | 李曼曼,姚日生,李凤和,胡华佳. 中药丹皮残渣固态发酵产酶与糖化工艺的研究[J]. 安徽化工, 2010, 36(Z1): 57-60. DOI: 10.3969/j.issn.1008-553X.2010.z1.026 |
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| 作者姓名： | 李曼曼 姚日生 李凤和 胡华佳 |
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| 作者单位： | 合肥工业大学化学工程学院制药工程系,安徽,合肥,230009;合肥工业大学化学工程学院制药工程系,安徽,合肥,230009;农产品生物化工教育部工程研究中心,安徽,合肥,230009 |
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| 摘 要： | 利用绿色木霉菌对丹皮提取残渣固态发酵产纤维素酶。以纤维素酶的酶活为指标,丹皮残渣与麸皮的比例、(NH4)2SO4添加量、发酵时间、接种量、发酵温度及固液比为考查因素,采取先单因素后正交实验的方法选取最佳发酵工艺。结果表明,最佳发酵条件为:丹皮残渣与麸皮的比例为2.5∶2.5,(NH4)2SO4添加量3%,发酵时间12天,接种量20%,发酵温度30℃,固液比1∶1.2。丹皮渣固态发酵残渣进行糖化发酵,糖化率为7.6%。因此,丹皮提取残渣固态发酵产纤维素酶是可行的,且发酵后残渣可以直接进行糖化发酵。
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| 关 键 词： | 中药渣 绿色木霉菌 固态发酵 纤维素酶 |
Production of Cellulase from Residues of the Peony Tree Root Bark by Solid-state Fermentation and the Saccharification |
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| LI Man-man,YAO Ri-sheng,LI Feng-he,HU Hua-jia. Production of Cellulase from Residues of the Peony Tree Root Bark by Solid-state Fermentation and the Saccharification[J]. Anhui Chemical Industry, 2010, 36(Z1): 57-60. DOI: 10.3969/j.issn.1008-553X.2010.z1.026 |
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| Authors: | LI Man-man YAO Ri-sheng LI Feng-he HU Hua-jia |
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| Affiliation: | LI Man-man1,YAO Ri-sheng1,2,LI Feng-he1,HU Hua-jia1 (1. School of Chemical Engineering,Hefei University of Technology,Hefei 230009,China,2. Engineering Research Center of Bioprocess of Ministry of Education,China) |
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| Abstract: | It is to research the technology of production of cellulase from TCM residue by Trichoderma viride ZY-1 in solid-state fermentation. The activity of cellulase is taken as the investigative target,through single-factor and orthogonal experiments,to study the effects on the cellulase activity of the ratio of residues of the peony tree root bark and wheat bran,content of(NH4)2SO4,fermentation time,inoculum size,fermentation temperature,and the solid-to-liquid ratio. The results showed that best technical condi... |
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| Keywords: | TCM residues Trichoderma viride solid-state fermentation cellulase |
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