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| 响应面法优化酿酒酵母乙醛脱氢酶和乙醇脱氢酶酶活检测方法 | |
| 引用本文： | 张彦青,贾士儒,张五九.响应面法优化酿酒酵母乙醛脱氢酶和乙醇脱氢酶酶活检测方法[J].中国酿造,2011(7). |
| 作者姓名： | 张彦青 贾士儒 张五九 |
| 作者单位： | 1. 天津科技大学工业微生物重点实验室,天津300457;中国食品发酵工业研究院,北京100027 2. 天津科技大学工业微生物重点实验室,天津,300457 3. 中国食品发酵工业研究院,北京,100027 |
| 摘 要： | 从酿酒酵母前处理方式方面对ALD和ADH酶活测定条件进行了优化,采用单因素试验对前处理方式进行了分析研究,结果表明破壁时间、超声波功率、超声时间、反应温度和缓冲液pH值对酶活测定值产生一定的影响.通过Box-Behnken中心组合设计和响应面分析法,确定了测定这2种酶的最理想酵母前处理条件,即超声功率420W,超声时间为11min,破碎时间为12min. |
| 关 键 词： | 酿酒酵母 乙醛脱氢酶 乙醇脱氢酶 响应面 优化 |
Optimization of assay method of aldehyde dehydrogenase and alcohol dehydrogenase activity in Saccharomyces cerevisiae by response surface methodology |
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| ZHANG Yanqing,JIA Shiru,ZHANG Wujiu.Optimization of assay method of aldehyde dehydrogenase and alcohol dehydrogenase activity in Saccharomyces cerevisiae by response surface methodology[J].China Brewing,2011(7). | |
| Authors: | ZHANG Yanqing JIA Shiru ZHANG Wujiu |
| Affiliation: | ZHANG Yanqing1,2,JIA Shiru1,ZHANG Wujiu2(1.Tianjin University of Science and Technology,Tianjin Key Laboratory of Industry Microbiology,Tianjin 300457,China,2.China National Research Institute of Food and Fermentation Industries,Beijing 100027,China) |
| Abstract: | Pretreatment conditions of aldehyde dehydrogenase(ALD) and alcohol dehydrogenase(ADH) activity assay method was optimized with single-factor experiment.The results showed that t broken cell wall time,power and treating time of ultrasonic,temperature and buffer pH value had effects on detection of ALD and ADH activity.By Box-Behnken central composite design and response surface analysis method,the optimal pretreatment conditions for determination of two enzymes of yeast were obtained as follows: ultrasonic p... |
| Keywords: | Saccharomyces cerevisiae aldehyde dehydrogenase alcohol dehydrogenase response surface method optimization |
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