| 复合诱变选育茁霉多糖高产菌株 |
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| 引用本文： | 冯印,苏安祥,王玉华. 复合诱变选育茁霉多糖高产菌株[J]. 中国酿造, 2011, 0(8) |
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| 作者姓名： | 冯印 苏安祥 王玉华 |
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| 作者单位： | 吉林农业大学食品科学与工程学院,吉林长春,130118 |
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| 基金项目： | 吉林省科技厅科技支撑计划(20080251) |
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| 摘 要： | 茁霉多糖具有许多优良的化学性质,可以作为中间体合成多种有经济价值的工业用化合物,是一种潜在的重要化工平台产品.以出芽短梗霉Y为出发菌株,通过紫外2轮、亚硝基胍3轮复合诱变,高蔗糖浓度(20％)平板筛选得到6株高产突变株,其中菌株N3茁霉多糖产量质量浓度达到37.84g/L,比出发菌株产量提高71.22％.
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| 关 键 词： | 茁霉多糖 紫外 亚硝基胍诱变 |
Screening of a strain with high pullulan productivity by compound mutagenesis |
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| FENG Yin,SU Anxiang,WANG Yuhua. Screening of a strain with high pullulan productivity by compound mutagenesis[J]. China Brewing, 2011, 0(8) |
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| Authors: | FENG Yin SU Anxiang WANG Yuhua |
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| Affiliation: | FENG Yin,SU Anxiang,WANG Yuhua(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | Pullulan has many excellent chemical properties,which can be used as an intermediate to synthesize a variety of industrial compounds.Aureobasidium pullulans was treated by UV for two generation and NTG for three generation mutagenesis.Six mutant stains were screened by high sucrose concentration(20%) plate,in which a mutant strain N3 with high pullulan productivity was obtained.The product of pullulan by this strain was up to 37.84g/L,which is 71.22% higher than that of the parent strain. |
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| Keywords: | pullulan UV nitrosoguanidine mutagenesis |
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