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| 原生质体融合技术选育高效菌株降解聚乙烯醇 | |
| 引用本文： | 廖劲松,郭勇,庄桂. 原生质体融合技术选育高效菌株降解聚乙烯醇[J]. 食品与生物技术学报, 2005, 24(2): 34-37 |
| 作者姓名： | 廖劲松 郭勇 庄桂 |
| 作者单位： | 1. 华南师范大学,生命科学学院,广东,广州,510631 2. 华南理工大学,食品与生物工程学院,广东,广州,510640 3. 河南工业大学,生物工程系,河南,郑州,450052 |
| 摘 要： | 从自然界中分离聚乙烯醇(PVA)的降解细菌，经紫外线诱变，得到两株具有单重抗药性的突变菌株S7(Str^s，Kan^r)和K15(Str^r，Kan^s)，两者对PVA的去除率分别达到52％和58％．将S7和K15作为亲本菌株进行原生质体融合，并对融合条件进行优化，融合子F4菌株对PVA去除率达到79．9％，将其培养成活性污泥后，PVA去除率可达87％，是普通活性污泥的三四倍． |
| 关 键 词： | 原生质体融合技术 聚乙烯醇 高效菌株 选育 活性污泥 紫外线诱变 去除率 PVA 降解细菌 突变菌株 亲本菌株 融合条件 自然界 抗药性 K15 融合子 |
| 文章编号： | 1673-1689(2005)02-0034-04 |
| 修稿时间： | 2004-02-26 |
The Breeding of High Efficient Strain with the Technique of Protoplast Fusion to Degrade Polyvinyl-Alcohol |
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| LIAO Jin-song~,GUO Yong~,ZHUANG Gui~. The Breeding of High Efficient Strain with the Technique of Protoplast Fusion to Degrade Polyvinyl-Alcohol[J]. Journal of Food Science and Biotechnology, 2005, 24(2): 34-37 | |
| Authors: | LIAO Jin-song~ GUO Yong~ ZHUANG Gui~ |
| Affiliation: | LIAO Jin-song~1,GUO Yong~2,ZHUANG Gui~3 |
| Abstract: | Bacteria with the ability to degrade Polyvinyl-Alcohol(PVA), were isolated from natural specimens. Two single resistant mutants, S7(Str~(s)Kan~(r)) and K15(Str~(r)Kan~(s)) were obtained by UV-irradiation. The S7 strain and the K15 strain could remove 52% and 58% of PVA, respectively. Then, using protoplast fusion technique with the two mutants as parent strains, the conditions of protoplast fusion were optimized.The fusion strain F4 could remove (79.9)% PVA in specimen and the active sludge cultured with F4 could remove 87% PVA in specimen. The PVA removing ability was about 3~4 times of that with ordinary active sludge. |
| Keywords: | protoplast fusion strain breeding PVA degradation |
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