| 皱皮木瓜腐败微生物的分离鉴定 |
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| 引用本文： | 黄荣,王晓丽,王兆升,李游,方双杰. 皱皮木瓜腐败微生物的分离鉴定[J]. 食品科学, 2021, 42(4): 161-166. DOI: 10.7506/spkx1002-6630-20191210-112 |
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| 作者姓名： | 黄荣 王晓丽 王兆升 李游 方双杰 |
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| 作者单位： | （1.山东农业大学食品科学与工程学院，山东?泰安 271018；2.山东农业工程学院食品科学与工程学院，山东?济南 250100） |
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| 基金项目： | 山东省重点研发计划项目(2018GNC113008);山东省林业科技创新项目(LYCX04-2018-21)。 |
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| 摘 要： | 为探明皱皮木瓜贮藏保鲜过程中腐败变质的原因,采用组织分离法对引起皱皮木瓜腐败的微生物进行分离纯化,初步分离出2株细菌和6株霉菌,未分离出酵母菌.回接实验结果表明共有2株细菌、3株霉菌能引起皱皮木瓜腐败.通过形态学及分子生物学对5株腐败微生物进行鉴定,结果表明:2株细菌分别为巨大芽孢杆菌(Bacillus megater...
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| 关 键 词： | 皱皮木瓜 腐败微生物 霉菌 细菌 鉴定 |
Isolation and Identification of Spoilage Microorganisms from the Fruit of Chaenomeles speciosa(Sweet)Nakai |
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| HUANG Rong,WANG Xiaoli,WANG Zhaosheng,LI You,FANG Shuangjie. Isolation and Identification of Spoilage Microorganisms from the Fruit of Chaenomeles speciosa(Sweet)Nakai[J]. Food Science, 2021, 42(4): 161-166. DOI: 10.7506/spkx1002-6630-20191210-112 |
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| Authors: | HUANG Rong WANG Xiaoli WANG Zhaosheng LI You FANG Shuangjie |
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| Affiliation: | (1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;2. College of Food Science and Engineering, Shandong Agriculture and Engineering University, Jinan 250100, China) |
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| Abstract: | The aim of this study was to explore the cause of spoilage in the fruit of Chaenomeles speciosa(Sweet)Nakai during storage.The tissue isolation method was used to isolate the microorganisms causing decay of C.speciosa.Two bacterial strains and six mold strains but no yeast strains were obtained.The results of back-inoculation test showed that both the bacterial strains and three of the mold strains caused rot of C.speciosa fruit.These strains were identified by morphological and molecular biology methods as Bacillus megaterium,B.pseudomycoides,Penicillium crustosum,Cladosporium velox and P.expansum,respectively.This study will provide theoretical support for controlling spoilage microorganisms in C.speciosa fruit during storage. |
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| Keywords: | Chaenomeles speciosa(Sweet)Nakai spoilage microorganism mold bacterium identification |
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