沙棘多糖分离纯化及抗氧化活性

分离纯化沙棘多糖(sea buckthorn(Hippophae rhamnoides)polysaccharides,SBP),并对其单糖组分、结构表征及体外抗氧化活性进行分析.通过水提醇沉、Sevag法除蛋白得到沙棘粗多糖,利用DEAE-52纤维素柱对其进行分离纯化得到中性多糖SBP-I和酸性多糖SBP-II、SB...

Isolation,Purification and Antioxidant Activity of Polysaccharides from the Fruit of Hippophae rhamnoides

Polysaccharides from the fruit of sea buckthorn(Hippophae rhamnoides)(SBP) were isolated and purified,and their monosaccharide composition,structural characteristics and antioxidant activities in vitro were analyzed.The crude polysaccharides,prepared by sequential water extraction,alcohol precipitation and protein removal by the Sevag method,were fractionated by DEAE-52 cellulose column chromatography into three fractions:neutral polysaccharide SBP-Ⅰ and acidic polysaccharide SBP-Ⅱ and SBP-Ⅲ.The results of monosaccharide composition analysis showed that SBP-Ⅰ was composed of arabinose,xylose,mannose,glucose and galactose with a molar ratio of 1.18:1:2.20:32.17:1.45,SBP-Ⅱ was composed of xylose,mannose,glucose and galactose with a molar ratio of 1:0.28:1.02:0.20,and SBP-Ⅲ was composed of xylose,glucose and galactose with a molar ratio of 1:2.15:0.28.Infrared spectroscopy showed that all of them had the characteristic absorption peaks of polysaccharides.Antioxidant tests in vitro showed that the crude polysaccharides and the three purified polysaccharides exhibited strong antioxidant activity in a concentration-dependent manner,ranked in decreasing order of antioxidant activity as follows:VC> SBP-Ⅲ> SBP-Ⅱ> SBP-Ⅰ> crude polysaccharides.