| 具有透明质酸酶抑制活性益生菌的体外筛选及鉴定 |
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| 引用本文： | 雷文平,周辉,陈绮,周杏荣,吴坤,汪家琦,刘成国. 具有透明质酸酶抑制活性益生菌的体外筛选及鉴定[J]. 食品科学, 2021, 42(2): 151-157. DOI: 10.7506/spkx1002-6630-20191104-038 |
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| 作者姓名： | 雷文平 周辉 陈绮 周杏荣 吴坤 汪家琦 刘成国 |
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| 作者单位： | （1.湖南农业大学食品科技学院，湖南 长沙 410128；2.食品科学与生物技术湖南省重点实验室，湖南 长沙 410128；3.澳优乳业（中国）有限公司，湖南 长沙 410200） |
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| 基金项目： | 湖南省战略新兴产业科技攻关项目(2017GK4032);国家自然科学基金面上项目(31571811)。 |
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| 摘 要： | 通过乳酸菌传统分离技术和益生菌体外评价,并结合透明质酸酶体外抑制实验,快速筛选出具有潜在抗过敏活性的益生菌.结果表明,从牛奶样品中分离的5株具有酸和胆盐耐受性的菌株表现出良好益生特性,其初步判定为安全菌株,所有菌株有较高透明质酸酶抑制率,结合相关研究表明其能够作为抗过敏益生菌筛选的指标之一.其中菌株L02和L15呈现出...
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| 关 键 词： | 益生菌 透明质酸酶抑制 益生特性 细胞表面特性 植物乳杆菌 |
Screening and Identification of Probiotics with Hyaluronidase Inhibitory Activity in Vitro |
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| LEI Wenping,ZHOU Hui,CHEN Qi,ZHOU Xingrong,WU Kun,WANG Jiaqi,LIU Chengguo. Screening and Identification of Probiotics with Hyaluronidase Inhibitory Activity in Vitro[J]. Food Science, 2021, 42(2): 151-157. DOI: 10.7506/spkx1002-6630-20191104-038 |
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| Authors: | LEI Wenping ZHOU Hui CHEN Qi ZHOU Xingrong WU Kun WANG Jiaqi LIU Chengguo |
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| Affiliation: | (1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha 410128, China; 3. Ausnutria Dairy (China) Co. Ltd., Changsha 410200, China) |
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| Abstract: | Probiotic strains were isolated by the traditional lactic acid bacterial isolation procedure and the in vitro probiotic potential evaluation test and rapidly screened for potential anti-allergic activity by the in vitro hyaluronidase inhibition test. The results showed that five probiotic strains tolerant to acid and bile salt and were isolated from milk samples, which were initially identified as safe strains, and all the strains displayed high hyaluronidase inhibitory activity, which could be considered as a selection criterion for probiotic strains with anti-allergic activity. Strains L02 and L15 showed significant(P < 0.05) in vitro tolerance, cell surface characteristics and hyaluronidase inhibitory activity, indicating that they have anti-allergic probiotic potential. The strains were identified by 16 S rDNA sequencing as Lactobacillus plantarum. |
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| Keywords: | probiotics hyaluronidase inhibitory activity probiotic properties cell surface properties Lactobacillus plantarum |
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