| 响应面法优化超声波提取山荆子抗氧化物质工艺 |
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| 引用本文： | 吕 娜,刘 阳,夏海洋 等.响应面法优化超声波提取山荆子抗氧化物质工艺[J].中国酿造,2014(4):52-55. |
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| 作者姓名： | 吕 娜 刘 阳 夏海洋 |
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| 作者单位： | [1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]浙江万里学院生物与环境学院,浙江宁波315100 |
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| 基金项目： | 浙江省科技厅公益项目（2010C32066）;吉林农业大学博士启动基金（201308） |
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| 摘 要： | 采用Box-Behnken响应面分析法研究超声波提取山荆子抗氧化物质的工艺条件。在单因素试验的基础上,研究乙醇体积分数、超声功率、提取时间对提取液抗氧化性的影响。建立该工艺的二次多项式模型。依据回归分析确定最优提取条件。结果表明:回归模型具有高度显著性。最佳提取工艺条件为乙醇体积分数57%,提取时间42min,超声功率为493W。此时提取液抗氧化性最强,亚铁还原能力(FRAP)值达最大值12 375.8。
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| 关 键 词： | 山荆子 FRAP法 抗氧化能力 响应面法 提取条件 |
Optimization of ultrasonic wave extraction of antioxidant substances from Malus baccata by response surface methodology |
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| LV Na,LIU Yang,XIA Haiyang,CUI Yanyan,SHEN Minghao,ZHANG Jie.Optimization of ultrasonic wave extraction of antioxidant substances from Malus baccata by response surface methodology[J].China Brewing,2014(4):52-55. |
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| Authors: | LV Na LIU Yang XIA Haiyang CUI Yanyan SHEN Minghao ZHANG Jie |
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| Affiliation: | 1.College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China; 2. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China) |
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| Abstract: | Box-Behnken response surface methodology was employed to optimize the ultrasonic-assisted extraction of antioxidant substances from Malus baccata. On the basis of single factor investigation, effects of ethanol volume fraction, ultrasonic power and extraction time on the antioxidant activity of the extract were investigated. A quadratic regression model equation which described the extraction process was fitted. The optimum ex- traction parameters were determined by the regression analysis and the fitted regression model had high statistical significance. The optimum extraction conditions were determined as follows： ethanol volume fraction 57%, ultrasonic time 42 min, ultrasonic power 493 W. Under these conditions, the extract showed highest antioxidant activity with the FRAP value of 12 375.8. |
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| Keywords: | Malus baccata FRAP assay antioxidant capacity response surface methodology extraction condition |
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