磷酸化处理对蚕蛹多肽结合钙能力的影响

为探索磷酸化处理对多肽结合钙能力的促进作用,以蚕蛹多肽为研究对象,采用不同种类和添加量的磷酸化试剂对其进行磷酸化改性,再与CaCl2反应制备蚕蛹多肽结合钙复合物,并对样品进行钙和磷含量测定、磷酸化位点检测、红外光谱分析和扫描电镜分析.研究结果表明:Na3 PO4、NaH2 PO4、(NaPO3)63种试剂可与蚕蛹多肽发...

Effect of Phosphorylation on Calcium-Binding Ability of Silkworm Pupa Polypeptide

To study the improvement of phosphorylation on calcium-binding ability of polypeptide,silkworm pupa polypeptide was phosphorylated by different kinds of phosphorylation reagents with diverse additive volume,then reacted with CaCl₂to prepare calcium-peptide complex.The content of calcium and phosphorus,phosphorylation site,FTIR and SEM of samples were analyzed.The results showed that phosphorylation occurred between polypeptide and Na₃PO₄,NaH₂PO₄,and(NaPO₃)₆,which led to the calcium content of the calcium-peptide complex enhanced from 145.12 mg/g to 202.32 mg/g.The calcium-binding ability was irrelated to the kinds of phosphorylation reagents,but related to the level of phosphorylation.The calcium content first increased then decreased with the increment of phosphorylation reagents.When the mass fraction of NaH₂PO₄was 0.50%,the binding calcium content reached maximum.Twenty-two increased phosphorylation sites were identified in 17 peptides after phosphorylation treatment by NaH₂PO₄.FTIR and phosphorylation site detection confirmed that phosphate groups were successfully grafted onto the peptide backbone through hydroxyl groups on serine and threonine.Phosphorylation could improve the calcium-binding ability of polypeptide,and provide new ideas and methods for development of effective calcium supplements.