姬松茸多糖提取物对高脂血症大鼠降血脂作用研究

为探讨姬松茸多糖对高脂血症大鼠降血脂作用及机制,采用水提醇沉法提取姬松茸粗多糖,并用DEAE-纤维素离子交换柱对多糖进行层析分级,采用高效液相色谱法分析单糖组成,并测定多糖含量;高脂饮食诱导建立高脂血症大鼠模型,将大鼠随机分成4组(空白对照组、模型组、辛伐他汀阳性对照组、姬松茸多糖组),同时对大鼠口服灌胃给药,连续42...

Lipid Lowering Effect of Agaricus blazei Polysaccharide Extract on Hyperlipidemia Rats

In order to investigate the effect and mechanism of Agaricus blazei Murill polysaccharide on reducing blood lipid in hyperlipidemic rats,the crude polysaccharide was extracted by water extraction and alcohol precipitation method,and the polysaccharide was graded by DEAE cellulose ion exchange column chromatography.The monosaccharide composition was analyzed by high performance liquid chromatography,and the content of polysaccharide was determined.The rats were randomly divided into four groups:blank control group,model group,simvastatin positive control group,Agaricus blazei Murill polysaccharide group,and oral administration for 42 days.The contents of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)in serum were detected by ELISA.HE staining method was used to observe the pathological changes of liver tissue,and the mRNA levels of LDL-R,SREBP-1C,CYP7a-1,PPAR-αand SCARB-1F were detected by RT-PCR.The results showed that the content of sugar in Agaricus blazei polysaccharide was 75.1%,and ABP-A contained 88.6 GLc,2.4%Gal,1.9%Man,1.0%Rha,0.5%GalA,1.9%Xyl,2.1%Ara,and 1.6%Fuc.ABP-A significantly decreased liver index(P<0.05),and serum TC,TG,LDL-C(P<0.01),but significantly increased HDL-C(P<0.01),lipid droplets deposition in liver was decreased by ABP-A.LDL-R,SCARB-1F mRNA expression(P<0.05)and CYP7a-1,PPAR-αmRNA levels(P<0.01)significantly increased,and SREBP-1C mRNA expression dramatically decreased(P<0.01).These results indicated that ABP-A exerted significant effects on lowering blood lipid,and the mechanism may be related to regulate the expression of LDL-R,SREBP-1c,CYP7a-1,PPAR-αand SCARB-1F.