基于核酸等温扩增的侧流层析试纸条在病原微生物检测中的研究进展

传统的聚合酶链反应(polymerase chain reaction, PCR)扩增方法具有较高的灵敏度、特异性和技术成熟性,但PCR仪成本高、操作复杂,且易出现假阳性。因此更为简单、便捷、低成本的基于核酸等温扩增的试纸条快速检测技术逐渐发展起来。目前,核酸等温扩增技术被应用于细菌、病毒等病原微生物的检测,其在食品安全中食源性致病菌的监管等方面也有着广阔的应用前景,因此受到广泛的关注。本文介绍了侧流层析技术的检测原理,总结了目前常用的核酸等温扩增技术及其在食源性致病菌检测方面的应用,并对其发展前景进行了展望,以便于掌握该技术的发展趋势,为相关学术研究提供研究思路。

Research progress of side flow chromatography strip based on isothermal amplification of nucleic acid in the detection of pathogenic microorganisms

Traditional PCR amplification method has high sensitivity, specificity and technical maturity, however, the PCR instrument is high in cost and complex in operation,it is prone to false positives. Therefore, a more simple, convenient and low-cost rapid detection technology based on nucleic acid isothermal amplification has gradually developed. At present, nucleic acid isothermal amplification technology has been applied to the detection of pathogenic microorganisms such as bacteria and viruses, and it also has broad application prospects in the supervision of foodborne pathogens in food safety, which has attracted extensive attention. In this paper, the detection principle of lateral flow strip was introduced, the commonly used nucleic acid isothermal amplification technology and its application in the detection of foodborne pathogens were summarized, and its development prospect was outlooked.It is easy to master the development trend of this technology and provide ideas for relevant academic research.